Catalysis of cis/trans isomerization in native HIV-1 capsid by human cyclophilin A

Proceedings of the National Academy of Sciences of the United States of America
Daryl A BoscoDorothee Kern

Abstract

Packaging of cyclophilin A (CypA) into HIV-1 virions is essential for efficient replication; however, the reason for this is unknown. Incorporation is mediated through binding to the Gly-89-Pro-90 peptide bond of the N-terminal domain of HIV-1 capsid (CA(N)). Despite the fact that CypA is a peptidyl-prolyl cis/trans isomerase, catalytic activity on CA(N) has not been observed previously. We show here, using NMR exchange spectroscopy, that CypA does not only bind to CA(N) but also catalyzes efficiently the cis/trans isomerization of the Gly-89-Pro-90 peptide bond. In addition, conformational changes in CA(N) distal to the CypA binding loop are observed on CypA binding and catalysis. The results provide experimental evidence for efficient CypA catalysis on a natively folded and biologically relevant protein substrate.

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Citations

Nov 11, 2006·Journal of Biomolecular NMR·Michael JohnGottfried Otting
Aug 20, 2010·Immunologic Research·Marisa S Briones, Samson A Chow
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