Catalytic activity of trypsin entrapped in electrospun poly(ϵ-caprolactone) nanofibers

Enzyme and Microbial Technology
Susana C PintoJ A Lopes-da-Silva

Abstract

Trypsin was successfully entrapped in situ into nanofibers of poly(ϵ-caprolactone) (PCL) prepared by electrospinning. The spinning dope was an emulsion consisting of an aqueous phase with the solubilized enzyme in a pH buffer plus an oil phase of the polymer solubilized in chloroform (CF)/dimethylformamide (DMF). The optimized materials were composed by random arrays of bead-free fibers with outer diameters in the range 110-180 nm without showing core-shell structure. The fiber size and morphology, membrane porosity and surface properties were shown to be influenced by the polymer concentration and the composition ratio of the solvent mixture, and also by the presence of the enzyme. The activity of the immobilized trypsin was studied toward both a low-molecular weight synthetic substrate (BAPNA) and a protein (casein). Fluorescence microscopy, the increasing hydrophilicity of the fibrous membrane and the observed catalytic activity confirmed the entrapment of the enzyme into the PCL nanofibers. The best activity retention (∼66% toward BAPNA) was achieved using 0.20 g/mL PCL in CF/DMF [75:25], with trypsin in an aqueous buffer at pH 7.1 in the presence of benzamidine and Span80. The immobilized enzyme showed satisfactory operati...Continue Reading

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Citations

Dec 19, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Shuhei KoedaToshihisa Mizuno
Nov 6, 2018·Critical Reviews in Biotechnology·Muhammad BilalHafiz M N Iqbal
Jan 1, 2016·Nanomaterials·Sichao HouMing Su
Sep 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chiara Maria Antonietta GangemiMaria Elena Fragalà
Nov 1, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Anabela MoreiraGareth R Williams
Jul 10, 2018·International Journal of Biological Macromolecules·Jaqueline Pozzada Dos SantosNathan Levien Vanier
Jan 6, 2019·International Journal of Biological Macromolecules·Mariana Dias Antunes PortoAlvaro Renato Guerra Dias
Jul 27, 2018·ACS Applied Materials & Interfaces·Anbharasi VanangamudiXing Yang
Oct 22, 2021·Biotechnology and Bioengineering·Anjum Hamid RatherFaheem A Sheikh

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