PMID: 40847Sep 1, 1979

Catalytic and regulatory properties of the Triton and trypsin forms of the brush border hydrolases

Gut
A M UgolevG G Koltushkina

Abstract

Amphipathic enzymes, invertase (EC 3.2.1.26), 8-amylase (EC 3.2.1.3), and alkaline phosphatase (EC 3.1.3.1), were purified from the rat small intestinal mucosa as trypsin and Triton forms, the catalytic and regulatory characteristics of which were compared in rats and in drosophila. Differences in the catalytic propertiis of the two enzyme forms were demonstrated, which suggested that the hydrophobic part of the enzyme was involved in maintaining optimal conformation of the catalytic part. Many modifiers have beenfound to influence the Triton rather than the trypsin form of the enzyme. It is therefore suggested that the hydrophobic sub-units of the enzymes might be involved in transmitting information from the cytoplasm into the external surface of the membrane, the cell in this way regulating the activity of surface enzymes. If this is indeed the case, it is suggested that the hydrophobic part performs functions not only of external but also of internal regulation.

References

Sep 1, 1975·The British Journal of Nutrition·A M UgolevG G Shcherbakov
Oct 17, 1975·Biochimica Et Biophysica Acta·H SigristG Semenza
Apr 5, 1976·Biochemical and Biophysical Research Communications·F PattusP Desnuelle
Nov 1, 1963·The Biochemical Journal·A DAHLQVIST, D L THOMSON
Jan 1, 1964·Analytical Biochemistry·A DAHLQVIST
Sep 1, 1972·Gut·A M Ugolev

Citations

Jan 1, 1983·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A M UgolevA A Grusdkov
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·V V EgorovaA M Ugolev
Jan 12, 2000·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·R I Kushak, H S Winter
Feb 25, 2010·The British Journal of Nutrition·Usha N S Prakash, Krishnapura Srinivasan
Jan 27, 2011·The British Journal of Nutrition·Supriya Veda, Krishnapura Srinivasan

Related Concepts

Alkaline Phosphatase
Amylases
Catalysis
Plasma Membrane
Drosophila melanogaster
Hydrogen-Ion Concentration
Hydrolase
Structure of Intestinal Gland
Microvilli
Vigilon

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