PMID: 8512926Jun 22, 1993

Catalytic centers in the thiamin diphosphate dependent enzyme pyruvate decarboxylase at 2.4-A resolution

Biochemistry
F DydaF Jordan

Abstract

The crystal structure of brewers' yeast pyruvate decarboxylase, a thiamin diphosphate dependent alpha-keto acid decarboxylase, has been determined to 2.4-A resolution. The homotetrameric assembly contains two dimers, exhibiting strong intermonomer interactions within each dimer but more limited ones between dimers. Each monomeric subunit is partitioned into three structural domains, all folding according to a mixed alpha/beta motif. Two of these domains are associated with cofactor binding, while the other is associated with substrate activation. The catalytic centers containing both thiamin diphosphate and Mg(II) are located deep in the intermonomer interface within each dimer. Amino acids important in cofactor binding and likely to participate in catalysis and substrate activation are identified.

Citations

May 1, 1995·Protein Science : a Publication of the Protein Society·M S HassonGeorge L Kenyon
Jul 1, 1995·Plant Molecular Biology·M BucherC Kuhlemeier
Jun 1, 2000·Journal of Inorganic Biochemistry·Gerasimos MalandrinosNick Hadjiliadis
Jul 10, 1998·Biochimica Et Biophysica Acta·A Schellenberger
Jul 10, 1998·Biochimica Et Biophysica Acta·Gerhard HübnerS Ghisla
Jul 10, 1998·Biochimica Et Biophysica Acta·U Schörken, G A Sprenger
Jul 10, 1998·Biochimica Et Biophysica Acta·H IdingM Pohl
Jul 10, 1998·Biochimica Et Biophysica Acta·David M ChipmanJ V Schloss
Dec 5, 2000·Current Opinion in Biotechnology·O P Ward, A Singh
Jan 30, 1999·The International Journal of Biochemistry & Cell Biology·G SchenkP F Nixon
Jul 25, 2006·Chemical Society Reviews·Gerasimos MalandrinosNick Hadjiliadis
Apr 15, 1996·European Journal of Biochemistry·U MückeS König
Aug 15, 1997·European Journal of Biochemistry·G SchenkRonald G Duggleby
Feb 1, 1995·Biological Chemistry Hoppe-Seyler·U MückeGerhard Hübner
Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·K MaM W Adams
Dec 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·N NemeriaF Jordan
Jan 8, 2008·Nature Chemical Biology·Alexander KaplunBoaz Shaanan
May 31, 2011·Nature Chemical Biology·Kyung-Jo LeeSun-Shin Cha
Dec 17, 2008·Organic & Biomolecular Chemistry·Karl M ErixonFinian J Leeper
May 30, 2009·Biochemical and Biophysical Research Communications·Matthew E C CainesChristopher J Schofield
Feb 1, 2008·Plant Physiology and Biochemistry : PPB·Ronald G DugglebyLuke W Guddat
Aug 6, 2005·IUBMB Life·German A Kochetov, I A Sevostyanova
Dec 7, 2007·Journal of Computational Chemistry·Mette Alstrup Lie, Birgit Schiøtt
Jun 11, 2002·Biochemical and Biophysical Research Communications·Marina V KovinaGerman A Kochetov
Aug 18, 2004·Archives of Biochemistry and Biophysics·Lioubov G KorotchkinaMulchand S Patel
Oct 18, 2005·Biochimica Et Biophysica Acta·Malea M KneenMichael J McLeish
May 30, 2015·Bioresource Technology·Praveen Kumar AgarwalSantosh B Noronha
Sep 16, 1999·Archives of Biochemistry and Biophysics·Lioubov G KorotchkinaMulchand S Patel
Sep 1, 2016·Applied Microbiology and Biotechnology·Yadi LiuXiaoyuan Wang
Oct 5, 1993·Biochemistry·M T Tse, J V Schloss
Jan 29, 2002·Journal of Molecular Biology·Matthew Bashton, Cyrus Chothia
Jan 25, 2017·Journal of Bacteriology·Sungmin HwangJulie A Maupin-Furlow
Aug 9, 2003·The Journal of Biological Chemistry·Guofeng ZhangDebra Dunaway-Mariano
Sep 1, 1994·European Journal of Biochemistry·M PohlM R Kula
Nov 11, 2014·Beilstein Journal of Organic Chemistry·Amjid IqbalFinian J Leeper
Mar 30, 2001·The Journal of Biological Chemistry·E FiedlerGerhard Hübner
Dec 24, 2002·The Journal of Biological Chemistry·Siew Siew PangRonald G Duggleby
Jul 13, 2018·Frontiers in Chemistry·Ferran PlanasFahmi Himo
Mar 13, 2019·Journal of Experimental Botany·Liem T BuiFrancesco Licausi
Jan 5, 2000·The Journal of Biological Chemistry·D I SvergunS König
Jan 22, 1998·Applied and Environmental Microbiology·P LuT W Jeffries
Jan 7, 2021·Applied Microbiology and Biotechnology·Pwj Labuschagne, B Divol

Related Concepts

Catalysis
In Silico
Pyruvate Decarboxylase
Saccharomyces
Berolase
X-Ray Diffraction
Protein Folding, Globular

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