Catalytic domain of the diversified Pseudomonas syringae type III effector HopZ1 determines the allelic specificity in plant hosts

Molecular Microbiology
Robyn L MorganWenbo Ma

Abstract

The type III secretion systems (T3SS) and secreted effectors (T3SEs) are essential virulence factors in Gram-negative bacteria. During the arms race, plants have evolved resistance (R) genes to detect specific T3SEs and activate defence responses. However, this immunity can be efficiently defeated by the pathogens through effector evolution. HopZ1 of the plant pathogen Pseudomonas syringae is a member of the widely distributed YopJ T3SE family. Three alleles are known to be present in P. syringae, with HopZ1a most resembling the ancestral allelic form. In this study, molecular mechanisms underlying the sequence diversification-enabled HopZ1 allelic specificity is investigated. Using domain shuffling experiments, we present evidence showing that a central domain upstream of the conserved catalytic cysteine residue determines HopZ1 recognition specificity. Random and targeted mutagenesis identified three amino acids involved in HopZ1 allelic specificity. Particularly, the exchange of cysteine141 in HopZ1a with lysine137 at the corresponding position in HopZ1b abolished HopZ1a recognition in soybean. This position is under strong positive selection, suggesting that the cysteine/lysine mutation might be a key step driving the evolu...Continue Reading

References

Jul 15, 1996·Nucleic Acids Research·J P VartanianS Wain-Hobson
Apr 1, 1997·Molecular Plant-microbe Interactions : MPMI·Lisa M KeithJ E Partridge
Nov 21, 1997·Computer Applications in the Biosciences : CABIOS·Z Yang
Dec 30, 1998·Trends in Biochemical Sciences·E A Van der Biezen, J D Jones
Feb 10, 2000·Molecular Biology and Evolution·Z Yang, R Nielsen
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·M A Martí-RenomA Sali
Nov 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·Mary Beth MudgettBrian J Staskawicz
Nov 28, 2000·Journal of Bacteriology·W GassmannBrian J Staskawicz
Feb 24, 2001·Plant Molecular Biology·M C Heath
Jul 19, 2001·Nature·Jeffery L Dangl, J D Jones
Aug 24, 2001·Biological Chemistry·A J Barrett, N D Rawlings
Feb 8, 2002·Trends in Plant Science·Renier A L Van der HoornMatthieu H A J Joosten
Feb 9, 2002·Current Opinion in Microbiology·Kim Orth
Jun 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Laurent DeslandesYves Marco
Nov 18, 2003·Molecular Microbiology·Andrew HotsonMary Beth Mudgett
Feb 6, 2004·The Plant Journal : for Cell and Molecular Biology·Yashitola JamirJames R Alfano
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Jean T. Greenberg
Jun 16, 2004·Molecular Plant-microbe Interactions : MPMI·Julie RodenMary Beth Mudgett
Jun 24, 2004·Infection and Immunity·James R Bretz, Steven W Hutcheson
Jul 31, 2004·Annual Review of Phytopathology·James R Alfano, Alan Collmer
Nov 18, 2004·Biochimica Et Biophysica Acta·Sheng Yang HeThomas S Whittam
Dec 14, 2004·Science·Rebecca L AllenJim L Beynon
Dec 20, 2005·Current Biology : CB·Andrew R PitmanDawn L Arnold
Dec 31, 2005·Nucleic Acids Research·N D RawlingsA J Barrett
Feb 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Robert B AbramovitchGregory B Martin
Feb 25, 2006·Cell·Stephen T ChisholmBrian J Staskawicz
May 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Peter N DoddsJeffrey G Ellis
Jun 7, 2006·Annual Review of Microbiology·Sarah R GrantJeffery L Dangl
Aug 29, 2006·Nature Reviews. Molecular Cell Biology·Robert B AbramovitchGregory B Martin
Nov 17, 2006·Nature·Jonathan D Jones, Jeffery L Dangl
Nov 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Rohit MittalHarvey T McMahon
Dec 1, 2006·Nature·Jorge E Galán, Hans Wolf-Watz
Oct 9, 2007·Molecular Plant-microbe Interactions : MPMI·Ioannis StergiopoulosPierre J G M De Wit
May 9, 2008·Nucleic Acids Research·Christian ColeGeoffrey J Barton
Jul 1, 2008·Current Opinion in Plant Biology·Wenbo Ma, David S Guttman
Aug 30, 2008·The Plant Cell·Renier A L Van der Hoorn, Sophien Kamoun
Jan 10, 2009·Molecular Plant-microbe Interactions : MPMI·Saskia A HogenhoutSophien Kamoun
Mar 11, 2009·Molecular Plant-microbe Interactions : MPMI·Eva H Stukenbrock, Bruce A McDonald
Aug 7, 2009·Molecular Plant-microbe Interactions : MPMI·Ming GuoJames R Alfano

Citations

Mar 30, 2011·Current Opinion in Plant Biology·Dawn L Arnold, Robert W Jackson
Jul 6, 2011·Molecular Plant Pathology·Jennifer D LewisDarrell Desveaux
Jun 20, 2014·The Plant Journal : for Cell and Molecular Biology·James WongWenbo Ma
Feb 11, 2015·The New Phytologist·Anna PiaseckaPaweł Bednarek
Aug 16, 2016·Nature Structural & Molecular Biology·Zhi-Min ZhangJikui Song
Jul 25, 2017·Nature Plants·Zhi-Min ZhangJikui Song
Oct 28, 2016·Microbiology and Molecular Biology Reviews : MMBR·Ka-Wai Ma, Wenbo Ma
May 2, 2018·Nature Communications·Kelley ClarkWenbo Ma

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