Catalytic hairpin assembly gel assay for multiple and sensitive microRNA detection

Theranostics
Wenhao DaiXueji Zhang

Abstract

As important modulators of gene expression, microRNAs (miRNAs) have been identified as promising biomarkers with powerful predictive value in diagnosis and prognosis for several diseases, especially for cancers. Here we report a facile, multiple and sensitive miRNA detection method that uses conventional gel electrophoresis and catalytic hairpin assembly (CHA) system without any complex nanomaterials or enzymatic amplification. Methods: In this study, three pairs of hairpin probes are rationally designed with thermodynamically and kinetically preferable feasibility for the CHA process. In the present of target miRNA, the stem of the corresponding hairpin detection probe (HDP) will be unfolded and expose the concealed domain. The corresponding hairpin assistant probe (HAP) then replaces the hybridized target miRNA to form specific HDP/HAP complexes and releases miRNA based on thermodynamically driven entropy gain process, and the released miRNA triggers the next recycle to produce tremendous corresponding HDP/HAP complexes. Results: The results showed that the CHA gel assay can detect miRNA at fM levels and shows good capability of discriminating miRNA family members and base-mismatched miRNAs. It is able to analyze miRNAs extra...Continue Reading

Citations

Feb 23, 2020·Beilstein Journal of Nanotechnology·María Sanromán Iglesias, Marek Grzelczak
Aug 21, 2019·Chemical Communications : Chem Comm·Wenhao DaiXueji Zhang
Nov 27, 2019·Molecular Aspects of Medicine·Guillaume GinesValerie Taly
Mar 2, 2021·Drug Discovery Today·Ruchita ShandilyaPradyumna Kumar Mishra
May 14, 2021·Biosensors & Bioelectronics·Ying PengGenxi Li
Feb 5, 2019·Chemical Reviews·Friedrich C SimmelHari R Singh

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Methods Mentioned

BETA
electrophoresis
PCR
fluorescence spectroscopy
biosensor

Software Mentioned

Matlab

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