Catalytic mechanism of type 2 isopentenyl diphosphate:dimethylallyl diphosphate isomerase: verification of a redox role of the flavin cofactor in a reaction with no net redox change

Biochemical and Biophysical Research Communications
Hisashi HemmiTokuzo Nishino

Abstract

Type 2 isopentenyl diphosphate:dimethylallyl diphosphate isomerase requires redox co-enzymes, i.e., flavin mononucleotide (FMN) and NAD(P)H, for activity, although it catalyzes a non-redox reaction. Spectrometric studies and enzyme assays under anaerobic conditions indicate that FMN is reduced through the reaction and is sufficient for activity. The sole function of NAD(P)H appears to be the reduction of FMN since it could be replaced by an alternate reducing agent. When the enzyme was reconstructed with a flavin analogue, no activity was detected, suggesting that the isomerase reaction proceeds via a radical transfer mechanism.

References

Oct 1, 1986·The Biochemical Journal·S Ghisla, V Massey
Nov 5, 1997·Science·J C Sacchettini, C D Poulter
Oct 24, 2000·Genome Research·A Smit, A Mushegian
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y MeahV Massey
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Felix RohdichWolfgang Eisenreich
Jan 11, 2003·Natural Product Reports·Stephen Bornemann
Mar 11, 2004·European Journal of Biochemistry·Satoshi YamashitaTokuzo Nishino
May 11, 2004·Nature Structural & Molecular Biology·Michelle Soltero-HigginLaura L Kiessling

❮ Previous
Next ❯

Citations

Nov 2, 2013·Chemical Reviews·Alexander R MoiseEleanore T Wurtzel
Jun 6, 2007·Journal of the American Chemical Society·Jonathan B JohnstonC Dale Poulter
Jun 22, 2007·The Journal of Organic Chemistry·Steven O MansoorabadiHung-wen Liu
Dec 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Takuya NagaiHisashi Hemmi
Jan 6, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Takeshi HoshinoTakashi Kumasaka
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Yosuke Koga, Hiroyuki Morii
Jun 12, 2013·Annual Review of Biochemistry·Lishan ZhaoPinghua Liu
Oct 12, 2007·Bioscience, Biotechnology, and Biochemistry·Takeshi Hoshino, Tadashi Eguchi
Jun 28, 2017·Archives of Biochemistry and Biophysics·Pablo Sobrado, John J Tanner
Oct 2, 2012·Chembiochem : a European Journal of Chemical Biology·Katharina DurchscheinKurt Faber
Dec 22, 2007·Annals of the New York Academy of Sciences·Laura L Grochowski, Robert H White
Mar 19, 2008·Journal of the American Chemical Society·Steven C RothmanC Dale Poulter
Nov 18, 2005·Progress in Lipid Research·Florence BouvierBilal Camara
Aug 3, 2013·Chemical Reviews·Philip D KiserKrzysztof Palczewski
Aug 8, 2007·Journal of Molecular Biology·Pier Federico GherardiniMichael J E Sternberg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.