Catalytic metalloporphyrin protects against paraquat neurotoxicity in vivo

Biomedical and Environmental Sciences : BES
Ping ChenLi-Ping Liang

Abstract

To examine the neuroprotective effects of a novel manganese porphyrin, manganese (III) meso-tetrakis (N,N'-diethylimidazolium-2-yl) porphyrin (MnTDM), in the mouse model of Parkinson's disease (PD) induced by paraquat (PQ). Male C57BL/6 mice were subcutaneously injected with either saline or PQ at 2-day intervals for a total of 10 doses, MnTDM was subcutaneously injected with the PQ 2 h before treatment. Performance on the pole and swim test were measured 7 days after the last injection and animals were sacrificed one day later. Levels of dopamine (DA) and its metabolites in the striatum were measured by high-performance liquid chromatography with an electrochemical detector (HPLC-ECD). Thiobarbituric acid (TBA) method was used to assay the lipid peroxidation product, malondialdehyde (MDA), and the number of tyrosine hydroxylase (TH) positive neurons was estimated using immunohistochemistry. Pretreatment with MnTDM significantly attenuated PQ-impaired behavioral performance, depleted dopamine content in striata, increased MDA, and dopaminergic neuron loss in the substantia nigra. Oxidative stress plays an important role in PQ-induced neurotoxicity which can be potentially prevented by manganese porphyrin. These findings also pr...Continue Reading

References

Mar 25, 2005·Neurosurgical Review·Hakan OzsüerSabahattin Cobanoğlu
Jul 29, 2005·Annals of Neurology·John P CrowM Flint Beal

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Citations

Nov 30, 2011·Antioxidants & Redox Signaling·Rommy von Bernhardi, Jaime Eugenín
Jan 15, 2011·Parkinson's Disease·Darcy LitteljohnShawn Hayley
May 28, 2013·Antioxidants & Redox Signaling·Huaxin ShengDavid S Warner
Jun 20, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Biswajit PodderHo-Yeon Song

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