PMID: 2113846Jan 1, 1990Paper

Catalytic properties and chemical composition of pepsins from Atlantic cod (Gadus morhua)

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
A GildbergJ B Bjarnason

Abstract

1. Three pepsins were purified from the gastric mucosa of Atlantic cod (Gadus morhua). 2. The enzymes, called Pepsin I and Pepsin IIa and b, had isoelectric points 6.9, 4.0 and 4.1, respectively, and digested hemoglobin at a maximal rate at a pH of approximately 3. 3. They resembled bovine cathepsin D in being unable to digest the mammalian pepsin substrate N-acetyl-L-phenylalanyl-3,5-diiodo-L-tyrosine. 4. Specificity constants (kcat/Km) for the cod pepsins were lower than for porcine pepsin, and they expressed higher substrate affinity and physiological efficiency at pH 3.5 than at pH 2. 5. The cod pepsins are glycoproteins, and their amino acid composition resembles that of porcine cathepsin D more than that of porcine pepsin. 6. The N-terminal sequence of Atlantic cod pepsins is substantially different from that of porcine pepsin. This indicates a significant evolutionary gap between fish and mammalian pepsins.

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Citations

Jan 1, 1990·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A Gildberg, K Overbø
Mar 21, 1998·Plastic and Reconstructive Surgery·M Signorini, G L Campiglio
Nov 28, 2007·Zhurnal evoliutsionnoĭ biokhimii i fiziologii·V A MukhinV Iu Novikov
Jan 14, 2012·International Journal of Otolaryngology·Karna Dev BardhanPeter W Dettmar
Mar 19, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Liang ChiJun Li
Dec 21, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Min-Jie CaoWen-Jin Su
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Oct 17, 2015·Food Chemistry·Mariusz Szymczak, Adam Lepczyński
Jan 22, 2015·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yoko MiuraAkihiko Moriyama
Dec 29, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Tadahide KurokawaTohru Suzuki
Sep 16, 1999·The Journal of Veterinary Medical Science·K AdachiK Ohwada
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