Catalytic superoxide scavenging by metal complexes of the calcium chelator EGTA and contrast agent EHPG

Biochemical and Biophysical Research Communications
A E O FisherD P Naughton

Abstract

Metal ion chelators widely used in experimental protocols and clinical diagnosis are generally assumed to be inert. We previously reported that the ubiquitous chelator EDTA has high levels of superoxide suppressing activity. Here, we report that the common chelators calcium chelator EGTA and contrast agent EHPG have significant activities in suppressing superoxide levels depending on the nature of metal ion chelated. The most active species is Mn(II)-EGTA which exhibited an IC50 value of 0.19 microM for superoxide destruction. In addition, IC50 values for Mn(II)-EHPG and 2Cu(II)-EGTA were 0.69 and 0.60 microM, respectively. In conclusion, Mn(II) and Cu(II) complexes of the common chelators EGTA and EHPG exhibit considerable superoxide scavenging activities. Caution should be employed in their use in biological systems where superoxide has a key role and they may be useful for the development of catalytic anti-oxidants.

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Citations

Mar 9, 2005·Biochemical and Biophysical Research Communications·Anna E O FisherDeclan P Naughton
Jan 26, 2010·Antioxidants & Redox Signaling·Ines Batinić-HaberleIvan Spasojević
Aug 6, 2009·BMC Complementary and Alternative Medicine·Tamsyn S A ThringDeclan P Naughton
Apr 14, 2005·Nutrition Journal·Anna E O Fisher, Declan P Naughton
Dec 11, 2007·Free Radical Biology & Medicine·Mohamed SamaiPrabal K Chatterjee
Sep 23, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xican LiDongfeng Chen
Jun 13, 2019·Biomedical Engineering Online·Alexander F SmithJuan M Jiménez
May 3, 2005·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·A E O Fisher, D P Naughton

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