Catchup: a mouse model for imaging-based tracking and modulation of neutrophil granulocytes

Nature Methods
Anja HasenbergMatthias Gunzer

Abstract

Neutrophil granulocyte biology is a central issue of immunological research, but the lack of animal models that allow for neutrophil-selective genetic manipulation has delayed progress. By modulating the neutrophil-specific locus Ly6G with a knock-in allele expressing Cre recombinase and the fluorescent protein tdTomato, we generated a mouse model termed Catchup that exhibits strong neutrophil specificity. Transgene activity was found only in very few eosinophils and basophils and was undetectable in bone marrow precursors, including granulomonocytic progenitors (GMPs). Cre-mediated reporter-gene activation allowed for intravital two-photon microscopy of neutrophils without adoptive transfer. Homozygous animals were Ly6G deficient but showed normal leukocyte cellularity in all measured organs. Ly6G-deficient neutrophils were functionally normal in vitro and in multiple models of sterile or infectious inflammation in vivo. However, Cre-mediated deletion of FcγRIV in neutrophils reduced the cells' recruitment to immune-complex-mediated peritonitis, suggesting a cell-intrinsic role for activating Fc receptors in neutrophil trafficking.

References

Jan 6, 2000·Transgenic Research·B E ClausenI Förster
Apr 12, 2001·BMC Developmental Biology·S SrinivasF Costantini
Jul 26, 2005·Immunity·Falk NimmerjahnJeffrey V Ravetch
Jun 21, 2007·The Journal of Biological Chemistry·Ulrich J H SachsSentot Santoso
Dec 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zoltán JakusAttila Mócsai
Sep 10, 2011·Nature Reviews. Immunology·Katherine Y King, Margaret A Goodell
Sep 16, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Emily R ElliottClifford A Lowell
Nov 2, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anna KleimanJan P Tuckermann
Jul 6, 2012·Nature·Prithu SunddKlaus Ley
Jan 3, 2013·Immunological Reviews·Mike HasenbergMatthias Gunzer
May 28, 2013·Nature·Tim LämmermannRonald N Germain
Jun 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Michaela SeelingFalk Nimmerjahn
Jul 13, 2013·Nature·Hong YangJulian R Thompson
Oct 22, 2013·British Journal of Haematology·Matthias Gunzer
Feb 6, 2014·Proceedings of the National Academy of Sciences of the United States of America·G-One AhnJ Martin Brown
May 27, 2014·Journal of Immunological Methods·Clare L AbramClifford A Lowell
Oct 13, 2014·Nature Immunology·Burkhard BecherEvan W Newell

❮ Previous
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Citations

Feb 26, 2016·Frontiers in Immunology·Enrica PietronigroGabriela Constantin
Jan 21, 2016·Proceedings of the National Academy of Sciences of the United States of America·Anna-Maria RolleStefan Wiehr
Mar 16, 2016·Seminars in Immunology·Catharina Hagerling, Zena Werb
Jun 10, 2015·Journal of Hepatology·Pedro Elias MarquesGustavo Batista Menezes
Jun 11, 2016·Nature Reviews. Cancer·Seth B CoffeltKarin E de Visser
Aug 2, 2016·Cell·Efstathios G StamatiadesFrederic Geissmann
Aug 26, 2016·Immunological Reviews·Korbinian Kienle, Tim Lämmermann
Feb 6, 2017·Autoimmunity Reviews·Courtney S CasserlyLuc Vallières
Nov 1, 2016·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Markus VaasJan Klohs
Mar 14, 2017·Nature Reviews. Immunology·Oliver SoehnleinChristian Weber
Apr 25, 2018·Zeitschrift für Rheumatologie·G SchettR H Straub
Jun 22, 2018·Proceedings of the National Academy of Sciences of the United States of America·Dana WestmeierRoland H Stauber
Jul 26, 2018·Journal of Leukocyte Biology·Carolyne K Smith, Giorgio Trinchieri
Nov 6, 2018·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·C L StothersJ K Bohannon
Jan 21, 2017·F1000Research·Justin F Deniset, Paul Kubes
Nov 18, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marc SchusterMatthias Gunzer
Aug 5, 2016·Journal of the American Society of Nephrology : JASN·Anika KlingbergMatthias Gunzer
May 28, 2019·Nature Communications·Simon F MerzMatthias Totzeck
Oct 17, 2019·Frontiers in Immunology·Matthias Bartneck, Jing Wang
Jan 28, 2020·Journal of Leukocyte Biology·Paige Lacy
Mar 15, 2020·Journal of Leukocyte Biology·William N T NguyenKamala D Patel
Mar 20, 2020·The Journal of Clinical Investigation·Tracie PlantSarah R Walmsley
Aug 20, 2020·Circulation Research·Ehsan VafadarnejadClément Cochain
Aug 16, 2019·Physiological Reviews·Carla Riera-DomingoMassimiliano Mazzone
Sep 25, 2020·The Journal of Experimental Medicine·Prarthana J DalalWilliam A Muller
Oct 5, 2018·The Journal of Experimental Medicine·Maria Casanova-AcebesAndrés Hidalgo
Feb 15, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Catherine A HawleyStephen J Jenkins
Jul 18, 2018·Nature Microbiology·Emma BoldockSimon J Foster
Dec 24, 2018·Acta Neuropathologica·Amaia Otxoa-de-AmezagaAnna M Planas
Jul 12, 2019·Frontiers in Immunology·Simon SodySven Brandau
Jul 23, 2019·The Journal of Clinical Investigation·Jennifer C BrazilCharles A Parkos

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