Catechol-Functional Chitosan/Silver Nanoparticle Composite as a Highly Effective Antibacterial Agent with Species-Specific Mechanisms

Scientific Reports
Xiaofei HuangQiaoling Hu

Abstract

In this study, silver nanoparticles (Ag NPs) coated with catechol-conjugated chitosan (CSS) were prepared using green methods. Interestingly, we uncovered that CSS-coated Ag NPs (CSS-Ag NPs) exhibited a higher toxicity against gram-negative Escherichia coli (E. coli) bacteria than against gram-positive Staphylococcus aureus (S. aureus) bacteria. The differences revealed that the CSS-Ag NPs killed gram bacteria with distinct, species-specific mechanisms. The aim of this study is to further investigate these underlying mechanisms through a series of analyses. The ultrastructure and morphology of the bacteria before and after treatment with CSS-Ag NPs were observed. The results demonstrated the CSS-Ag NPs killed gram-positive bacteria through a disorganization of the cell wall and leakage of cytoplasmic content. In contrast, the primary mechanism of action on gram-negative bacteria was a change in membrane permeability, induced by adsorption of CSS-Ag NPs. The species-specific mechanisms are caused by structural differences in the cell walls of gram bacteria. Gram-positive bacteria are protected from CSS-Ag NPs by a thicker cell wall, while gram-negatives are more easily killed due to an interaction between a special outer membran...Continue Reading

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Citations

Mar 7, 2018·Advanced Healthcare Materials·Leon Chien-Wei LinChe-Ming Jack Hu
Feb 23, 2019·Materials·Alexandra Muñoz-BonillaMarta Fernández-García
May 16, 2019·Advanced Healthcare Materials·Eunsook ParkHaeshin Lee
Sep 14, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ana C AlmeidaNatália M Alves
Dec 4, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Sovan Lal BanerjeeNikhil K Singha
Jul 28, 2018·Antibiotics·Jorge H LeitãoMaria Fernanda N N Carvalho
Jun 18, 2020·Materials·Dominika Czerwińska-GłówkaKatarzyna Krukiewicz
Nov 27, 2020·Nanomaterials·Oana GherasimAlexandru Mihai Grumezescu
Jan 12, 2021·Pharmaceutical Development and Technology·Wipada SamprasitBenchawan Chamsai
Jan 27, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Seyedehfatemeh RazaviamriBruce P Lee
Jun 10, 2021·Environmental Science and Pollution Research International·Fang LiuCan Kong
Jun 26, 2021·Scientific Reports·Ulviye BunyatovaJennifer Zhang
Aug 24, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Ameya R NarkarKollbe Ahn
Jun 22, 2018·Biomacromolecules·Chunhua CaoGregory F Payne
Nov 5, 2020·Carbohydrate Polymers·Gemma C PorterCarla J Meledandri

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Methods Mentioned

BETA
electron microscopy
protein assay
surface plasmon resonance
X-ray

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