PMID: 36171Mar 22, 1979

Catecholamine and guanine nucleotide activation of skeletal muscle adenylate cyclase

Biochimica Et Biophysica Acta
P Nambi, G I Drummond


Activation of adenylate cyclase by guanine nucleotide and catecholamines was examined in plasma membranes prepared from rabbit skeletal muscle. The GTP analog, 5'-guanylyl imidodiphosphate caused a time and temperature-dependent activation of the enzyme which was persistent, the Ka was 0.05 microM. 5'-Guanylyl imidodiphosphate binding to the membranes was time and temperature dependent, KD 0.07 microM. Beta adrenergic amines accelerated the rate of 5'-guanylyl imidodiphosphate activation of the enzyme with an order of potency isoproterenol approximately soterenol approximately salbutamol greater than epinephrine greater than norephrine. Catecholamine activation was antagonized by propranolol and the beta2 antagonist butoxamine; the beta1 antagonist practolol was inactive. [3H]Dihydroalprenolol bound to the membranes and binding was antagonized by beta adrenergic agonists with an order of potency similar to the activation of adenylate cyclase and was antagonized by butoxamine but not by practolol. The data are consistent with the idea that adenylate cyclase in skeletal muscle plasma membranes is coupled to adrenergic receptors of the beta2 type.


May 1, 1980·Naunyn-Schmiedeberg's Archives of Pharmacology·S ShimaM Asakura
Apr 9, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·C CerriL P Rowland
Oct 1, 1985·Archives of Biochemistry and Biophysics·J R Mickelson, C F Louis
Feb 1, 1983·Biochemical Pharmacology·M Wright, G I Drummond
Sep 10, 2005·The Journal of Physiology·Danielle S BattramFlemming Dela
Apr 15, 1980·Archives of Biochemistry and Biophysics·G I DrummondP Nambi
Jun 1, 1986·The Journal of Applied Bacteriology·D ReynoldsP Hambleton

Related Concepts

Tissue Membrane
Catecholamine [EPC]
Antagonist Muscle Action
Guanylyl Imidodiphosphate
Catecholamines Measurement
Adrenergic beta-Agonists
Epinephrine Measurement
Adrenergic Receptor

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