Catecholamine assays in a rat model for autonomic dysreflexia

Archives of Physical Medicine and Rehabilitation
F Y Chiou-TanG C Chiou

Abstract

To determine if norepinephrine (NE) levels increased during autonomic dysreflexia (AD) hypertension in spinal cord injured rats. Prospective, randomized study of three groups of eight rats that underwent C8 spinal cord transection. Two days postoperatively, catecholamine samples were collected from the animals as follows: group 1 without preoperative chemical sympathectomy using 6-hydroxydopamine (6-OH DA) or induced AD; group 2 with induced AD; and group 3, preoperative chemical sympathectomy with 6-OH DA and induced AD. Basic science research laboratory in an academic institution. Sprague-Dawley male rats weighing 300g. Three episodes of induced AD hypertension with bowel distension for 1 minute. Results showed a significant difference between groups 1 and 2, 2 and 3, and 1 and 3 (p=.0002, ANOVA, Y1/2, post hoc Tukey). The mean NE levels in groups 1, 2, and 3 were 465+/-400 ng/mL, 1,328+/-1,116 ng/mL, and 31+/-61 ng/mL, respectively. NE increased during AD in the spinal cord injured rat. NE levels were effectively blocked by 6-OH DA when AD was induced. Knowledge of catecholamine levels in this process may assist in determining both pathophysiology and potential pharmacologic treatment options in future human studies.

References

Mar 1, 1983·Journal of the Autonomic Nervous System·C J Mathias, H L Frankel
Jul 1, 1994·American Journal of Physical Medicine & Rehabilitation·F Y Chiou-TanM Grabois
Dec 1, 1947·Brain : a Journal of Neurology·L GUTTMANN, D WHITTERIDGE

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Citations

Jul 30, 2002·Autonomic Neuroscience : Basic & Clinical·Samuel Leman, Henrique Sequeira
Jul 27, 1999·American Journal of Physical Medicine & Rehabilitation·F Y Chiou-TanC S Robertson
Jan 22, 2009·Avian Pathology : Journal of the W.V.P.A·Ulrike FoldenauerJean-Michel Hatt
Nov 30, 2019·Frontiers in Cellular Neuroscience·Felicia M MichaelAlexander G Rabchevsky

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