Catecholamines and the kinetics of lipolysis in isolated rat adipocytes. Statistical analysis and handling of day-to-day variability in dose-response curves: a general procedure for assessing and manipulating dose-response data

Biochemical Pharmacology
J I DaviesD Everett

Abstract

(1) As a first step in studying the kinetics of the lipolytic system of rat adipocytes, the day-to-day variation between dose-response curves has been analysed. (2) Methods are described for the evaluation of large quantities of data relating noradrenaline to lipolysis. (3) A 'clustering' technique is presented which can be used both to estimate and minimise differences between curves along the response axis. Criteria are outlined to determine whether clustering is appropriate. (4) Our findings indicate that the basic form of the relationship between lipolysis and noradrenaline concentration is relatively stable and consequently that the data can be utilized to study the relationship in greater detail. (5) The techniques described should be applicable to all hormone-mediated responses and may therefore provide the first step towards a meaningful analysis of the relationship between hormone concentrations and response in many systems.

Related Concepts

Fat Pad
Metazoa
Catecholamines
Dose-Response Relationship, Drug
Lipolysis
Norepinephrine, (+, -)-Isomer
August Rats
Rats, Laboratory
In Vitro [Publication Type]

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