Cathepsin B activates human trypsinogen 1 but not proelastase 2 or procarboxypeptidase B

Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]
Björn LindkvistAnders Borgström

Abstract

Activation of trypsinogen to trypsin is a crucial step in the development of acute pancreatitis. The cause of this activation is not known although suggested explanations include autoactivation, cathepsin B-mediated activation and activation by mast cell tryptase. The aim of this study was to investigate cathepsin B and tryptase activation of pancreatic zymogens. Trypsinogen-1, proelastase, and procarboxypeptidase B were purified from human pancreatic juice. Human cathepsin B and betaI-tryptase are commercial products. Activation and degradation of zymogens were measured by activity towards specific substrates for trypsin and pancreatic elastase, ELISAs for procarboxypeptidase B and its activation peptide, and a radioimmunoassay for the trypsinogen activation peptide. Cathepsin B caused activation of trypsinogen-1 with a trypsin yield of about 30% of that produced by enterokinase. Proelastase and procarboxypeptidase B was not activated by cathepsin B. None of the zymogens were inactivated by cathepsin B. Neither monomeric nor tetrameric tryptase could activate any of the examined zymogens. Cathepsin B is a competent activator of trypsinogen-1, although not as efficient as enterokinase. If cathepsin B is to play a role in protea...Continue Reading

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Citations

Nov 26, 2010·Journal of Gastroenterology and Hepatology·Minoti V ApteJeremy S Wilson
Dec 3, 2014·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Vivek MishraVijay P Singh
Jan 9, 2013·Expert Opinion on Therapeutic Targets·Christopher S Gondi, Jasti S Rao
Feb 10, 2009·Scandinavian Journal of Gastroenterology·Roland AnderssonJinglin Xia
May 13, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Quang T LeLawrence B Schwartz
Jun 27, 2019·Scientific Reports·Alexandra DemcsákMiklós Sahin-Tóth
Oct 13, 2016·Annual Review of Pharmacology and Toxicology·Shawn M Davidson, Matthew G Vander Heiden

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