Cathepsin B-like proteinase is involved in the decomposition of the adult fat body of Helicoverpa armigera

Archives of Insect Biochemistry and Physiology
Xiao-Mei YangXiao-Fan Zhao

Abstract

Cathepsin B-like proteinase (HCB, EC 3.4.22.1) is expressed in Helicoverpa armigera oocytes and adult fat bodies. Previous work has revealed that HCB plays a key role in the degradation of yolk proteins during embryogenesis. This study investigated the function and regulatory activation of HCB in adult fat bodies during aging and oogenesis. The HCB transcript was detected at all stages from larval to adult fat bodies with Northern blot analysis. Pro-HCB was also detected in fat bodies at these stages with an immunoblot assay using a monoclonal antibody against HCB. However, mature HCB and its activity were only detected in fat bodies of pre-adults and adults. This evidence suggested that HCB is regulated post-translationally by activation of the pro-enzyme during the pupa-adult metamorphosis. The activation of HCB was coupled with the expression of hormone receptor 3 (HHR3), and was up-regulated by the ecdysteroid agonist, RH-2485, suggesting that HCB activation is related to the ecdysone regulatory system. The decomposition of the adult fat bodies during aging and oogenesis was found to occur via programmed cell death, in which HCB took part.

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Citations

Jun 2, 2007·Molecular Biology Reports·Dantong YangXiaofan Zhao
May 1, 2010·Toxins·Robert L Harrison, Bryony C Bonning
Apr 10, 2008·Insect Molecular Biology·Y D KooK Zhu-Salzman
Jan 28, 2017·Gene·Hai-Yan YanHideki Kawasaki
Apr 10, 2017·Genome Biology and Evolution·Elina ImmonenGöran Arnqvist
Mar 17, 2007·Archives of Insect Biochemistry and Physiology·Xiao-Mei YangXiao-Fan Zhao
Jan 1, 2008·Archives of Insect Biochemistry and Physiology·Alejandro RabossiLuis A Quesada-Allué
Aug 14, 2012·Insect Molecular Biology·X Zhai, X-F Zhao
Feb 15, 2011·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Xiaomei WangJianhai Xiang
Jun 23, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kwang Sik LeeByung Rae Jin

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