Cathepsin B secretion by rabbit articular chondrocytes: modulation by cycloheximide and glycosaminoglycans

Cell and Tissue Research
A Baici, A Lang

Abstract

Rabbit articular chondrocytes in monolayer culture are modulated away from their differentiated state and undergo morphological and biochemical changes. One of the characteristics of the modulated state is an abnormally high production of the cysteine endopeptidase cathepsin B. Addition to chondrocyte cultures of the protein biosynthesis inhibitor, cycloheximide, resulted in a concentration-dependent reduction of cathepsin B secretion, which was fully restored after removal of cycloheximide. Glycosaminoglycans added to the culture medium of modulated chondrocytes partially reduced the rate of secretion of cathepsin B, this effect being dependent on their structure, the degree of sulfation, and concentration. The age of the chondrocytes and the duration of the treatment also influenced this response. The switching off of cathepsin B release was apparently best favored by a high concentration of negatively charged sulfate groups attached to a polymeric glycosaminoglycan chain; this simulates the natural environment of the chondrocytes in articular cartilage.

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Citations

Jan 1, 1992·Rheumatology International·A BaiciF J Wagenhäuser
Aug 1, 1994·Clinica Chimica Acta; International Journal of Clinical Chemistry·V LecomteM Raes
Sep 1, 1999·European Journal of Biochemistry·K NisslerB Wiederanders
Oct 1, 1992·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·A TrabandtS Gay
Jan 1, 1994·Critical Reviews in Biochemistry and Molecular Biology·S S Twining
Jun 18, 2010·The FEBS Journal·Patricia Schenker, Antonio Baici
Apr 20, 2010·Osteoarthritis and Cartilage·J Martel-PelletierJ-P Pelletier
May 11, 2010·The Biochemical Journal·Marko NovinecAntonio Baici
Aug 15, 2001·Seminars in Arthritis and Rheumatism·J P BaliE Neuzil
Oct 7, 2005·Seminars in Arthritis and Rheumatism·Antonio BaiciKathrin Müntener
Jul 1, 2020·Terapevticheskiĭ arkhiv·V N DrozdovA K Starodubtsev

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