Cathepsin G cleaves and activates IL-36γ and promotes the inflammation of psoriasis

Drug Design, Development and Therapy
Jing GuoZhiQiang Yin

Abstract

IL-36γ is considered to be a valuable biomarker in psoriatic patients, which is expressed as an inactive precursor that needs to be proteolytically processed and activated, and neutrophil-derived proteases seemed to be potent activating enzymes of IL-36γ. This study aims to investigate the activation of IL-36γ by cathepsin G (CG) and neutrophil elastase (NE). We used inactive recombinant full-length (FL)-IL-36γ with different doses of NE or CG to stimulate HaCaT cells; neutrophil extracellular traps (NETs) were prepared to act on FL-IL-36γ and then stimulate HaCaT cells. Real-time quantitative PCR and ELISA were performed to detect CXCL-1 and CXCL-8 expression. We developed imiquimod-induced psoriasis-like mouse model to evaluate the effect of hypodermic injection of neutrophil-derived protease or its inhibitor. Histopathology and Western blotting were conducted for effect assessment. Purified CG cleaved and activated recombinant human FL-IL-36γ to promote CXCL-1 and CXCL-8 expression by human keratinocytes, and NETs activated FL-IL-36γ and the activation was inhibited by serpin A3. CG induced expression of a more truncated IL-36γ in psoriasiform lesion of mice and aggravated the psoriasis-like lesion induced by imiquimod, wher...Continue Reading

Citations

Mar 16, 2019·International Journal of Molecular Sciences·Marie-Astrid BoutetCostantino Pitzalis
Apr 30, 2020·Life Science Alliance·Jérémie D GoldsteinCem Gabay
Oct 28, 2019·Frontiers in Immunology·Chih-Chao ChiangTsong-Long Hwang
Nov 7, 2019·Technology in Cancer Research & Treatment·Deli MaoXin Zhao
Feb 5, 2021·Expert Review of Clinical Immunology·Helena Iznardo, Lluís Puig
Jan 28, 2021·Immunology·Vu L NgoTimothy L Denning
May 1, 2021·International Journal of Molecular Sciences·Helena Iznardo, Lluís Puig
May 27, 2021·International Reviews of Immunology·Abraham U Morales-PrimoJaime Zamora-Chimal
Jul 14, 2021·The Journal of Investigative Dermatology·Shuichiro NakaboMariana J Kaplan
Aug 28, 2021·International Journal of Molecular Sciences·Dominik SamotijAdam Reich

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
ELISA
blood draws

Software Mentioned

GraphPad
GraphPad Prism

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here