Cathepsin G enhances human natural killer cytotoxicity

Immunology
T Yamazaki, Y Aoki

Abstract

Cathepsin G is a serine protease located in the azurophil granules of neutrophils. In this study, we investigated the effect of cathepsin G on the functions of human natural killer (NK) cells in vitro. Cathepsin G enhanced NK cytotoxicity rapidly in a dose-dependent fashion. The ability to augment NK cytotoxicity was markedly reduced in the presence of the inhibitor, phenylmethanesulphonyl fluoride (PMSF) or chymostatin, demonstrating that the proteolytic activity of cathepsin G is essential for the induction of NK cytotoxicity. Granulocyte exocytosis is required for NK cell-dependent target killing. Cathepsin G induced the release of the granule enzyme, N-acetyl-beta-D-glucosaminidase, from human NK cells. Moreover, an increase in the cytosolic-free Ca2+ concentration was observed in NK cells after stimulation with cathepsin G. When human granulocytes were stimulated with cytochalasin B and N-formyl-methionyl-leucyl-phenylalanine (fMLP), cathepsin G was released. The cathepsin G released from granulocytes also caused enhancement of NK cytotoxicity. In the presence of serine protease inhibitor the supernatant including cathepsin G obtained from stimulated granulocytes did not enhance NK cytotoxicity, but the stimulated granuloc...Continue Reading

References

May 1, 1976·The Biochemical Journal·P M Starkey, A J Barrett
Jan 1, 1991·Annual Review of Immunology·E R PodackM G Lichtenheld
Jan 1, 1990·Annual Review of Immunology·J Tschopp, M Nabholz
Jan 1, 1989·Advances in Immunology·G Trinchieri
Sep 1, 1974·Hoppe-Seyler's Zeitschrift für physiologische Chemie·W Schmidt, K Havemann
May 28, 1981·Nature·C S HenneyS Gillis
Jun 1, 1982·The Journal of Clinical Investigation·Y AokiK Oshimi
Apr 7, 1995·Cell·G Berke
Sep 1, 1995·Immunology Today·J Brock
Feb 1, 1995·Current Opinion in Immunology·P Scott, G Trinchieri
Jan 1, 1995·Cellular Immunology·T Hase-Yamazaki, Y Aoki
Feb 1, 1996·Immunology Today·L L Lanier, J H Phillips
Jan 1, 1997·Journal of Leukocyte Biology·T Yamazaki, Y Aoki

❮ Previous
Next ❯

Citations

Mar 10, 2001·European Journal of Immunology·M B DelgadoM Wolf
Sep 16, 2006·Rheumatology International·Junya MiyataSaburo Sone
Feb 13, 2007·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·K-F HsuC-Y Chou
Sep 11, 2010·International Immunopharmacology·V Kumar, A Sharma
Jul 26, 2002·Microbial Pathogenesis·Yosuke Aoki, Tac Takeda
Aug 5, 2010·Journal of Leukocyte Biology·Claudio Costantini, Marco A Cassatella

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

© 2022 Meta ULC. All rights reserved