Cathepsin K mRNA detection is restricted to osteoclasts during fetal mouse development

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
R A DoddsM Gowen

Abstract

We recently identified a novel cysteine protease, cathepsin K, by random sequencing of an osteoclast cDNA library, and in situ hybridization studies in adult human tissues demonstrated high and specific expression in osteoclasts. To determine whether the expression of cathepsin K mRNA during mouse embryogenesis was more widespread, cryostat sections of early (day 11-13) and late (day 15-17) mouse fetuses were analyzed by in situ hybridization. Serial cross-sections were collected through each fetus, and co-reacted for tartrate-resistant acid phosphatase (TRAP) and nonspecific esterase (NSE), selective markers for the osteoclast, and precursor cells derived from the macrophage/monocyte lineage, respectively. In the 11-13 day fetuses, cathepsin K mRNA was not expressed in any extraskeletal tissue; at this stage of embryogenesis, no osteoclasts are present. However, in the 15-17 day fetuses, a distinctive, developmental stage-dependent pattern of cathepsin K expression was observed in osteoclasts and preosteoclasts at sites of cartilage and bone modeling. Cathepsin K positive osteoclasts differentiated within a peripheral zone of the osteogenic stacked cell layer of the cartilage rudiments (prior to ossification), migrated and/or ...Continue Reading

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Citations

Jan 28, 2009·Calcified Tissue International·Adele L BoskeyMitchell B Schaffler
Sep 22, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M GowenI Kola
May 11, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y P Li, W Chen
Nov 26, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y NishiB D Gelb
Oct 12, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y KobayashiH Sakai
Oct 5, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M ZaidiE Abe
Mar 30, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R A DoddsM Gowen
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