Cathepsin K preferentially solubilizes matured bone matrix

Calcified Tissue International
Olivier BorelPatrick Garnero

Abstract

Bone collagen undergoes a series of enzymatic and nonenzymatic posttranslational modifications with maturation. The aim of this study was to analyze the collagenolytic efficiency of cathepsin K in relation to the extent of bone collagen age. Bone collagen posttranslational maturation was induced in vitro by preincubating bovine fetal cortical bone specimens at 37 °C for different times. The collagen enzymatic cross-links pyridinoline (PYD) and deoxypyridinoline (DPD), the advanced glycation end product pentosidine (PEN), and the native (α) and β-isomerized C-telopeptide (CTX) isomers were measured in each bone specimen. After extraction, bone collagen was incubated with human recombinant cathepsin K at different concentrations and its collagenolytic activity was measured by the release of hydroxyproline. To assess the affinity of cathepsin K for isomerized and nonisomerized CTX isomers, incubation with cathepsin K was also performed in the presence of various concentrations of a specific inhibitor. We showed that preincubation of bone collagen at 37 °C induces a marked increase in the bone concentration of PYD, DPD, and PEN and of CTX isomerization as reflected by the ratio of α-/βCTX. This increase was associated with a parall...Continue Reading

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Citations

Jan 1, 2012·BoneKEy Reports·Patrick Garnero
Dec 1, 2015·Frontiers in Medicine·Adhyatmika AdhyatmikaBarbro N Melgert
Mar 31, 2016·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·E CavalierJ-J Body
Jan 15, 2015·BioMed Research International·Marko NovinecBoris Turk
Nov 20, 2016·FEBS Letters·Marko NovinecBrigita Lenarčič
Apr 22, 2015·Calcified Tissue International·Patrick Garnero
Jun 7, 2019·Journal of Cancer Research and Clinical Oncology·Weiping LiangJian Zhang
May 1, 2013·Biological Chemistry·Marko Novinec, Brigita Lenarčič
Dec 17, 2015·American Journal of Physiology. Endocrinology and Metabolism·Ziqing LiPuyi Sheng
Jun 25, 2014·The Journal of Biological Chemistry·Peter A LemaireLe T Duong
Apr 8, 2021·Protein Engineering, Design & Selection : PEDS·Debi Choudhury, Sampa Biswas
Aug 8, 2021·International Journal of Molecular Sciences·Yongguang GaoJingxian Jia

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