Abstract
Cathepsin L (Ctsl) plays a pivotal role in lysosomal and autophagic proteolysis. Previous investigations revealed that partial hepatectomy (PH) decreases biosynthesis of cathepsins in liver, followed by suppression of lysosomal and autophagic proteolysis during liver regeneration. Conversely, it was reported that autophagy-deficiency suppressed liver regeneration. Thus, the purpose of this study is to determine if Ctsl deficiency affects liver regeneration after PH. 70% of PH was performed in male Ctsl-deficient mice (Ctsl-/-) and wild-type littermates (Ctsl +/+) after PH. Mice were sacrificed and wet weight of the whole remaining liver was measured. Bromodeoxyuridine (BrdU)-immunostaining of liver sections was performed. Expression of cyclin D1, p62, LC-3, Nrf2, cleaved-Notch1, Hes1 was evaluated by western blot analysis. NQO1 mRNA expression was measured by realtime-PCR. After a 70% of PH, the liver mass was significantly restored within 5 days in Ctsl-/- mice compared to wild-type. Ctsl-deficiency enhanced the increases in both the rate of BrdU-positive cells and cyclin D1 expression after PH more than wild-type mice. On the other hand, Ctsl-deficiency upregulated p62, cleaved-Notch1 and Hes1 expression after PH. Moreover, t...Continue Reading
References
Jun 29, 1979·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·U Pfeifer
May 9, 1998·Science·T NakagawaA Y Rudensky
Oct 30, 1998·Journal of Biochemistry·K WatanabeE Kominami
Apr 2, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Christoph KöhlerGeorge K Michalopoulos
May 5, 2004·Journal of Hepatology·Shinji TogoHiroshi Shimada
Dec 18, 2007·Cell·Masaaki KomatsuKeiji Tanaka
Jan 24, 2009·Cellular and Molecular Life Sciences : CMLS·T Borggrefe, F Oswald
Mar 3, 2009·Biological & Pharmaceutical Bulletin·Katsuyuki TakahashiEiki Kominami
May 29, 2009·The Journal of Biological Chemistry·Boris Turk, Vito Turk
Oct 2, 2009·Carcinogenesis·Thomas W Kensler, Nobunao Wakabayashi
Dec 15, 2010·Cardiovascular Research·Mei SunPeter P Liu
Apr 13, 2011·The Journal of Cell Biology·Yoshihiro InamiMasaaki Komatsu
Aug 23, 2011·Biochemical and Biophysical Research Communications·Yoshihiro InamiSumio Watanabe
Mar 9, 2012·Trends in Cell Biology·Peter AndersenChulan Kwon
Aug 14, 2012·Cell Metabolism·John D Hayes, Michael L J Ashford
Dec 12, 2012·Journal of Cell Science·Daniel FisherBéla Novák
Mar 5, 2013·Nature·Maho HamasakiTamotsu Yoshimori
Apr 24, 2013·Journal of the American Heart Association·Mei SunPeter P Liu
Dec 5, 2013·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Yuka FukuoSumio Watanabe
Mar 22, 2014·Trends in Biochemical Sciences·John D Hayes, Albena T Dinkova-Kostova
Mar 29, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Takeo ToshimaYoshihiko Maehara
Sep 16, 2014·PloS One·Yuhong ZouGuoli Dai
Dec 20, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yuhong ZouGuoli Dai
May 25, 2015·Free Radical Biology & Medicine·Nobunao WakabayashiThomas W Kensler