Cathepsin L maturation and activity is impaired in macrophages harboring M. avium and M. tuberculosis

International Immunology
Rajeev M NepalPaula Bryant

Abstract

Mycobacterium tuberculosis-infected macrophages demonstrate diminished capacity to present antigens via class II MHC molecules. Since successful class II MHC-restricted antigen presentation relies on the actions of endocytic proteases, we asked whether the activities of cathepsins (Cat) B, S and L-three major lysosomal cysteine proteases-are modulated in macrophages infected with pathogenic Mycobacterium spp. Infection of murine bone marrow-derived macrophages with either Mycobacterium avium or M. tuberculosis had no obvious effect on Cat B or Cat S activity. In contrast, the activity of Cat L was altered in infected cells. Specifically, whereas the 24-kDa two-chain mature form of active Cat L predominated in uninfected cells, we observed an increase in the steady-state activity of the precursor single-chain (30 kDa) and 25-kDa two-chain forms of the enzyme in cells infected with either M. avium or M. tuberculosis. Pulse-chase analyses revealed that maturation of nascent, single-chain Cat L into the 25-kDa two-chain form was impaired in infected macrophages, and that maturation into the 24-kDa two-chain form did not occur. Consistent with these data, M. avium infection inhibited the IFNgamma-induced secretion of active two-chai...Continue Reading

References

May 1, 1989·Journal of Cellular Biochemistry·A H Erickson
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·B FineschiJ Miller
Aug 18, 1995·Journal of Medicinal Chemistry·J T PalmerD Brömme
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·V Y ReddyS J Weiss
Aug 1, 1994·Infection and Immunity·J GerckenH D Flad
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G F McIntyre, A H Erickson
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·J DeussingJ A Villadangos
Oct 1, 1998·European Journal of Immunology·P R WolfH L Ploegh
Nov 14, 1998·Biochemical and Biophysical Research Communications·K IshidohE Kominami
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·M E McGrath
Apr 18, 2000·Infection and Immunity·I Guérin, C de Chastellier
Nov 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·H J UllrichD G Russell
Feb 24, 2001·European Journal of Cell Biology·W L BeattyD G Russell
Mar 13, 2001·Annual Review of Immunology·J L Flynn, J Chan
Jul 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·E H NossC V Harding
Aug 3, 2001·The EMBO Journal·A M Lennon-DuménilP Wolf Bryant
Jan 29, 2002·European Journal of Immunology·Esther B E PlügerChristoph Driessen
Mar 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chyi-Song HsiehAlexander Y Rudensky
Jun 25, 2002·Advances in Immunology·Paula Wolf BryantHidde L Ploegh
Aug 21, 2002·The Journal of Experimental Medicine·Ana-Maria Lennon-DuménilCécile Lagaudrière-Gesbert
Nov 6, 2002·The Journal of Experimental Medicine·Edda FiebigerAna-Maria Lennon-Duménil
Jan 23, 2003·The Journal of Experimental Medicine·Courtney BeersAlexander Rudensky
Apr 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Rutilio A FrattiVojo Deretic
May 31, 2003·The Journal of Biological Chemistry·Kanae Shirahama-NodaIkuko Hara-Nishimura
May 31, 2003·Nature Reviews. Immunology·Karen Honey, Alexander Y Rudensky
Jun 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rish K PaiClifford V Harding
Jun 24, 2003·Biochimica Et Biophysica Acta·Kensaku OhashiEmiko Sano
Aug 20, 2003·The Journal of Experimental Medicine·Isabelle VergneVojo Deretic

❮ Previous
Next ❯

Citations

Mar 4, 2008·American Journal of Respiratory Cell and Molecular Biology·Konrad StadlerAldo Baritussio
Nov 26, 2013·Molecular Brain·Paula M HeronTimothy S McClintock
Aug 13, 2009·Atherosclerosis·K L MattockA Smith
Sep 29, 2015·Health Policy and Planning·Manuela ColombiniUNKNOWN ADVANCE Study Team
Aug 31, 2016·Scientific Reports·David PiresElsa Anes
Apr 11, 2017·Scientific Reports·Giorgio CasaburiJamie S Foster
Jul 20, 2014·Molecular & Cellular Proteomics : MCP·Tyler R SchleicherSpencer V Nyholm
Jul 2, 2008·Journal of Leukocyte Biology·Rajeev Mani NepalPaula Bryant
Sep 1, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kerstin WalterStefan Ehlers
Dec 22, 2020·Frontiers in Cellular and Infection Microbiology·Lidia Szulc-DąbrowskaFelix N Toka
Jun 25, 2015·Microbiology Spectrum·Michael F GoldbergSteven A Porcelli

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.