Cathepsin L of the sea cucumber Apostichopus japonicus-molecular characterization and transcriptional response to Vibrio splendidus infection

Fish & Shellfish Immunology
Jingwen YangTianming Wang

Abstract

Cathepsin L, a lysosomal endopeptidase, has been noted for its involvement in the innate immune response in invertebrates. Here, the cathepsin L cDNA of the sea cucumber Apostichopus japonicus (AjCatL) is identified from an EST library and then cloned by the rapid amplification of the cDNA ends (RACE) PCR. The full-length cDNA is 1678 bp long containing an open reading frame (ORF) of 1002 bp, an 80 bp 5' UTR and a 599 bp 3' UTR. The cDNA encodes 333 amino acid residues with a predicted molecular mass of 37.07 kDa and a theoretical isoelectric point (pI) of 5.01. The full-length AjCatL contains three active sites of eukaryotic thiol (cysteine) protease at positions 133-144, 278-288 and 295-314. Analysis of the predicted tertiary structure of prepro-CatL (17-333 aa) and mature-CatL (116-333 aa) reveals that the propeptide region (17-115 aa) blocks access to the substrate-binding cleft. Phylogenetic analysis shows that the AjCatL is clustered together with two other CatLs from Strongylocentrotus purpuratus. The enzymatic activity of AjCatL was verified using a substrate hydrolyzing assay with recombinant mAjCatL. Further analysis of real time-PCR demonstrates that the expression of AjCatL mRNA is significantly up-regulated in the ...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 30, 2003·Biological Chemistry·Kazumi Ishidoh, Eiki Kominami
Nov 23, 2005·Bioinformatics·Konstantin ArnoldTorsten Schwede
Dec 31, 2005·Nucleic Acids Research·Neil D RawlingsAlan J Barrett
Sep 11, 2007·Biochimie·Klaudia BrixSilvia Jordans
Jul 22, 2010·Swiss Medical Weekly·Sébastien Conus, Hans-Uwe Simon
Oct 6, 2010·The Journal of Clinical Investigation·Jochen ReiserThomas Reinheckel
Jul 20, 2011·International Journal of Oncology·Kohtaro MiyamotoSeiichi Takenoshita
Dec 12, 2012·Bioscience, Biotechnology, and Biochemistry·A Ram LeeHyung Ho Lee

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Citations

Jun 5, 2019·Journal of the Science of Food and Agriculture·Zi-Qiang LiuFereidoon Shahidi

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