Catheter colonization and abscess formation due to Staphylococcus epidermidis with normal and small-colony-variant phenotype is mouse strain dependent.

PloS One
Gunnar SanderEsther Mahabir

Abstract

Coagulase-negative staphylococci (CoNS) form a thick, multilayered biofilm on foreign bodies and are a major cause of nosocomial implant-associated infections. Although foreign body infection models are well-established, limited in vivo data are available for CoNS with small-colony-variant (SCV) phenotype described as causative agents in implant-associated infections. Therefore, we investigated the impact of the Staphylococcus epidermidis phenotype on colonization of implanted PVC catheters and abscess formation in three different mouse strains. Following introduction of a catheter subcutaneously in each flank of 8- to 12-week-old inbred C57BL/6JCrl (B6J), outbred Crl:CD1(ICR) (CD-1), and inbred BALB/cAnNCrl (BALB/c) male mice, doses of S. epidermidis O-47 wild type, its hemB mutant with stable SCV phenotype, or its complemented mutant at concentrations of 10(6) to 10(9) colony forming units (CFUs) were gently spread onto each catheter. On day 7, mice were sacrificed and the size of the abscesses as well as bacterial colonization was determined. A total of 11,500 CFUs of the complemented mutant adhered to the catheter in BALB/c followed by 9,960 CFUs and 9,900 CFUs from S. epidermidis wild type in BALB/c and CD-1, respectively....Continue Reading

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Citations

Oct 4, 2014·Clinical Microbiology Reviews·Karsten BeckerGeorg Peters
Feb 2, 2019·The Journal of Microbiology·Sergio Martínez-GarcíaJuan C Cancino-Diaz
Aug 1, 2018·Infection and Immunity·Kimberly Perez, Robin Patel
Mar 11, 2016·Clinical Microbiology Reviews·Barbara C KahlBettina Löffler
Jul 24, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Agnieszka Bogut, Agnieszka Magryś

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Methods Mentioned

BETA
biopsy

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