Cation-Activated Avidity for Rapid Reconfiguration of DNA Nanodevices

ACS Nano
Alexander E MarrasCarlos E Castro

Abstract

The ability to design and control DNA nanodevices with programmed conformational changes has established a foundation for molecular-scale robotics with applications in nanomanufacturing, drug delivery, and controlling enzymatic reactions. The most commonly used approach for actuating these devices, DNA binding and strand displacement, allows devices to respond to molecules in solution, but this approach is limited to response times of minutes or greater. Recent advances have enabled electrical and magnetic control of DNA structures with sub-second response times, but these methods utilize external components with additional fabrication requirements. Here, we present a simple and broadly applicable actuation method based on the avidity of many weak base-pairing interactions that respond to changes in local ionic conditions to drive large-scale conformational transitions in devices on sub-second time scales. To demonstrate such ion-mediated actuation, we modified a DNA origami hinge with short, weakly complementary single-stranded DNA overhangs, whose hybridization is sensitive to cation concentrations in solution. We triggered conformational changes with several different types of ions including mono-, di-, and trivalent ions an...Continue Reading

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Citations

Jul 28, 2020·Advanced Science·Yaqin HuYanfei Liu
Dec 5, 2019·Nucleic Acids Research·Ze Shi, Gaurav Arya
Dec 9, 2020·Sensors·Shuang WangYe Tian
Dec 16, 2020·Angewandte Chemie·Joonas RyssyAnton Kuzyk
Jul 13, 2021·Frontiers in Chemistry·Peng LinTakashi Morii
Oct 31, 2019·Nano Letters·Joshua A JohnsonCarlos E Castro
Jan 7, 2022·Chemistry, an Asian Journal·Jing WangDe-Ming Kong

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