Cation diffusion facilitator Cis4 is implicated in Golgi membrane trafficking via regulating zinc homeostasis in fission yeast

Molecular Biology of the Cell
Yue FangTakayoshi Kuno

Abstract

We screened for mutations that confer sensitivities to the calcineurin inhibitor FK506 and to a high concentration of MgCl(2) and isolated the cis4-1 mutant, an allele of the gene encoding a cation diffusion facilitator (CDF) protein that is structurally related to zinc transporters. Consistently, the addition of extracellular Zn(2+) suppressed the phenotypes of the cis4 mutant cells. The cis4 mutants and the mutant cells of another CDF-encoding gene SPBC16E9.14c (we named zrg17(+)) shared common and nonadditive zinc-suppressible phenotypes, and Cis4 and Zrg17 physically interacted. Cis4 localized at the cis-Golgi, suggesting that Cis4 is responsible for Zn(2+) uptake to the cis-Golgi. The cis4 mutant cells showed phenotypes such as weak cell wall and decreased acid phosphatase secretion that are thought to be resulting from impaired membrane trafficking. In addition, the cis4 deletion cells showed synthetic growth defects with all the four membrane-trafficking mutants tested, namely ypt3-i5, ryh1-i6, gdi1-i11, and apm1-1. Interestingly, the addition of extracellular Zn(2+) significantly suppressed the phenotypes of the ypt3-i5 and apm1-1 mutant cells. These results suggest that Cis4 forms a heteromeric functional complex with ...Continue Reading

References

Sep 1, 1992·Molecular and Cellular Biology·D S ConklinC Kung
Mar 1, 1992·The Journal of Cell Biology·D E Levin, E Bartlett-Heubusch
Jan 1, 1991·Methods in Enzymology·S MorenoP Nurse
Jan 1, 1983·Methods in Enzymology·R J Rothstein
Feb 23, 1995·Nature·R HeimR Y Tsien
Jun 3, 1997·Gene·S L Forsburg, D A Sherman
Apr 5, 2000·Biochimica Et Biophysica Acta·L E WilliamsJ L Hall
Dec 14, 2001·The Journal of Biological Chemistry·Dmitry NamgaladzeVolker Ullrich
Feb 8, 2002·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·L A Gaither, D J Eide
Jun 7, 2002·The Journal of Biological Chemistry·Gilles P M BorrellySimon K Whitehall
Aug 6, 2002·The Journal of Biological Chemistry·Colin W MacDiarmidDavid J Eide
Nov 26, 2002·The Journal of Biological Chemistry·Catherine P Kirschke, Liping Huang
Jan 31, 2003·The Journal of Biological Chemistry·Colin W MacDiarmidDavid J Eide
Feb 5, 2003·Biochemical and Biophysical Research Communications·Masaki Edamatsu, Yoko Y Toyoshima
Jun 28, 2003·FEMS Microbiology Reviews·Dietrich H Nies
Jan 6, 2004·Cellular and Molecular Life Sciences : CMLS·T KambeM Nagao
Jul 28, 2004·The Journal of Cell Biology·Charissa D EllisDavid J Eide
Jun 18, 2005·The Journal of Biological Chemistry·Charissa D EllisDavid J Eide
Feb 18, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yi HeTakayoshi Kuno
May 13, 2006·Genomics, Proteomics & Bioinformatics·Taiho KambeYuko Yamaguchi-Iwai
Jun 9, 2006·The Journal of Biological Chemistry·Attila KumánovicsJerry Kaplan
Jun 24, 2006·The Journal of Biological Chemistry·Robert J CousinsLouis A Lichten

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Citations

Jun 15, 2011·Bioscience, Biotechnology, and Biochemistry·Taiho Kambe
Jun 4, 2016·Fungal Genetics and Biology : FG & B·Lingling HuYue Fang
Jul 1, 2017·Metallomics : Integrated Biometal Science·Yarden GolanYehuda G Assaraf
May 14, 2011·Metallomics : Integrated Biometal Science·Toshiyuki Fukada, Taiho Kambe
Mar 21, 2020·Critical Reviews in Biochemistry and Molecular Biology·David J Eide
Mar 18, 2020·The Journal of Biological Chemistry·Eisuke SuzukiTaiho Kambe
May 23, 2018·Oxidative Medicine and Cellular Longevity·Teruaki TakasakiReiko Sugiura
Sep 18, 2009·The Journal of Biological Chemistry·Ayako FukunakaTaiho Kambe
Jun 27, 2019·The Journal of Biological Chemistry·Ya-Mei HuAmanda J Bird
Jan 25, 2021·The Journal of Biological Chemistry·Taiho KambeDax Fu
Nov 9, 2020·Journal of Plant Physiology·Yaohui WangZhi Qi
Feb 1, 2021·The Journal of Biological Chemistry·Eisuke SuzukiTaiho Kambe

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