PMID: 4883012Aug 1, 1968Paper

Cation fluxes and permeability changes accompanying bacteriophage infection of Escherichia coli

Journal of Virology
S SilverP M Spielman

Abstract

Infection of Escherichia coli by bacteriophage T2 was accompanied by a rapid but transient increase in the rate of loss of small molecules from the bacterial cells. This transient leakage was studied with radioactive labels such as (42)K and (28)Mg. Bacteriophage-induced leakage was dependent on the ratio of phage to bacteria: the higher the multiplicity of infection, the greater the leakage. No leakage occurred at 4 C [when adsorption proceeds but injection of phage deoxyribonucleic acid (DNA) is blocked]. Leakage was caused by heavily irradiated phage as well as by normal phage; therefore, the intracellular functioning of the bacteriophage DNA was not required. This conclusion was supported by experiments which showed phage-induced leakage in the presence of chloramphenicol or sodium cyanide. Leakage could be prevented by infecting the bacteria with phage in the presence of high magnesium concentrations. Phage-induced leakage was terminated by a "sealing" reaction, after which potassium turnover by infected and uninfected cells was very similar. The sealing reaction occurred even in the presence of chloramphenicol, suggesting that the sealing is controlled by bacterial and not bacteriophage genes. We were not able to detect a...Continue Reading

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Citations

Aug 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Bo HuJun Liu
Oct 1, 1996·Clinical Orthopaedics and Related Research·D G CampbellR D Oakeshott
Feb 21, 2008·Molecular Microbiology·Sundari ChodavarapuJon M Kaguni
Feb 1, 1984·Journal of Bioenergetics and Biomembranes·B Labedan, L Letellier
Jul 27, 1999·Virology·T KaiT Yonesaki
Dec 1, 1970·Journal of Bacteriology·S SilverK King
Jul 1, 1975·Journal of Virology·P S KhandekarM Chakravorty
Jun 1, 1969·Bacteriological Reviews·D A Marvin, B Hohn
Jan 1, 1974·Journal of Virology·G FletcherC F Earhart
Dec 1, 1982·Journal of Bacteriology·D B Wilson, A Okabe
Jul 1, 1971·Journal of Virology·M L Freedman, R E Krisch
Jun 1, 1984·Journal of Virology·C Hulen, J Legault-Demare
Dec 1, 1975·Bacteriological Reviews·K G Hardy
Aug 1, 1997·Journal of Bacteriology·R DaugelaviciusD H Bamford
Feb 1, 1978·Journal of Virology·S Thompson, J S Wiberg
May 1, 1969·Journal of Virology·R H Peterson, C S Buller
Oct 1, 1972·Journal of Bacteriology·E Eisenstadt
Jun 1, 1994·Trends in Microbiology·T Alatossava
Jul 26, 1976·Biochemical and Biophysical Research Communications·P N Bandyopadhyay, M Chakravorty

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