Cation-selective pathway of OmpF porin revealed by anomalous X-ray diffraction.

Journal of Molecular Biology
Balasundaresan DhakshnamoorthyBenoît Roux

Abstract

The OmpF porin from the Escherichia coli outer membrane folds into a trimer of beta-barrels, each forming a wide aqueous pore allowing the passage of ions and small solutes. A long loop (L3) carrying multiple acidic residues folds into the beta-barrel pore to form a narrow "constriction zone". A strong and highly conserved charge asymmetry is observed at the constriction zone, with multiple basic residues attached to the wall of the beta-barrel (Lys16, Arg42, Arg82 and Arg132) on one side, and multiple acidic residues of L3 (Asp107, Asp113, Glu117, Asp121, Asp126, Asp127) on the other side. Several computational studies have suggested that a strong transverse electric field could exist at the constriction zone as a result of such charge asymmetry, giving rise to separate permeation pathways for cations and anions. To examine this question, OmpF was expressed, purified and crystallized in the P6(3) space group and two different data sets were obtained at 2.6 A and 3.0 A resolution with K(+) and Rb(+), respectively. The Rb(+)-soaked crystals were collected at the rubidium anomalous wavelength of 0.8149 A and cation positions were determined. A PEG molecule was observed in the pore region for both the K(+) and Rb(+)-soaked crystal...Continue Reading

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Citations

Sep 26, 2012·Biochemistry Research International·Elena García-GiménezVicente M Aguilella
Feb 14, 2013·The EMBO Journal·Ludovic SauguetMarc Delarue
Dec 21, 2012·Proteins·Christof KattnerMikio Tanabe
Dec 4, 2012·Structure·Brigitte K Ziervogel, Benoît Roux
Jan 2, 2016·Biochimica Et Biophysica Acta·Karunakar R PothulaUlrich Kleinekathöfer
Dec 30, 2018·Journal of Biomolecular Structure & Dynamics·Maximilien Lopes-RodriguesEric A Perpète
Dec 7, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Vicente M AguilellaAntonio Alcaraz
Nov 4, 2010·Physical Chemistry Chemical Physics : PCCP·María Queralt-MartínAntonio Alcaraz
Sep 8, 2016·Veterinary Research·Luisa Samaniego-BarrónMireya de la Garza
Mar 17, 2011·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Carles CaleroMarcel Aguilella-Arzo
Jan 25, 2020·Biochimie·Sathishkumar MunusamyCarlos Munoz-Garay
Mar 17, 2021·Chemical Reviews·Jigneshkumar Dahyabhai PrajapatiMathias Winterhalter
Jun 18, 2021·Journal of Chemical Theory and Computation·Abhishek AcharyaUlrich Kleinekathöfer
Jan 28, 2012·The Journal of Physical Chemistry. B·Jorge NumataErnst-Walter Knapp
Oct 11, 2013·Journal of the American Chemical Society·Balasundaresan DhakshnamoorthyBenoît Roux
Nov 22, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·María Queralt-MartínAntonio Alcaraz
Dec 2, 2021·ACS Synthetic Biology·Sol Vendrell-FernándezRafael Giraldo
Apr 25, 2012·Journal of Structural Biology·D BalasubramaniamS Krishnaswamy

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