PMID: 45782Jan 1, 1978

Cation-sugar cotransport in the melibiose transport system of Escherichia coli

Membrane Biochemistry
T Tsuchiya, T H Wilson

Abstract

The entry of Na+ or H+ into cells of Escherichia coli via the melibiose transport system was stimulated by the addition of certain galactosides. The principal cell used in these studies (W3133) was a lactose transport negative strain of E. coli possessing an inducible melibiose transport system. Such cells were grown in the presence of melibiose, washed, and incubated in the presence of 25 microM Na+. The addition of thiomethylgalactoside (TMG) resulted in a fall in Na+ concentration in the incubation medium. No TMG-stimulated Na+ movement was observed in uninduced cells. In an alpha-galactosidase negative derivative of W3133 (RA11) a sugar-stimulated Na+ uptake was observed in melibiose-induced cells on the addition of melibiose, thiodigalactoside, methyl-alpha-galactoside, methyl-beta-galactoside, and galactose, but not lactose. It was inferred from these studies that the substrates of the melibiose system enter the cell on the melibiose carrier associated with the simultaneous entry of Na+ when this cation is present in the incubation medium. Extracellular pH was measured in unbuffered suspensions of induced cells in order to study proton movement across the membrane of cells exposed to different galactosides. In the absence...Continue Reading

References

Feb 19, 2000·Biochemical and Biophysical Research Communications·P Z Ding, T H Wilson
Jan 1, 1995·World Journal of Microbiology & Biotechnology·G SpeelmansW N Konings
Jan 1, 1989·Archives of Microbiology·H EbbighausenR Krämer
Aug 1, 1989·Biochimie·G LeblancM Bassilana
Mar 1, 1990·Research in Microbiology·M C BotfieldT H Wilson
Nov 14, 1998·Biochimica Et Biophysica Acta·T Hastings Wilson, D M Wilson
Dec 31, 1997·Biochimica Et Biophysica Acta·N OkazakiT Tsuchiya
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Mohammad S Yousef, Lan Guan
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T PourcherG Leblanc
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·W K Versaw, R L Metzenberg
Dec 15, 2010·The Journal of Biological Chemistry·Lan GuanSiva P Ankeshwarapu
Mar 19, 2002·Antimicrobial Agents and Chemotherapy·Takashi KatsuKeiko Yasuda
Jan 1, 1980·Membrane Biochemistry·T TsuchiyaT H Wilson
Jan 1, 1985·Membrane Biochemistry·D M WilsonT H Wilson
May 1, 2007·Experimental Physiology·David T Thwaites, Catriona M H Anderson
Aug 9, 2003·Microbiology and Immunology·Md Nazmul HudaTomofusa Tsuchiya
Sep 6, 2005·Journal of Structural Biology·Pasi PurhonenHans Hebert
Dec 1, 1991·Bioscience Reports·P J Henderson
Oct 17, 2007·FEMS Microbiology Letters·Chong Wai LiewNazalan Najimudin
Jun 28, 1985·Biochemical and Biophysical Research Communications·M BassilanaG Leblanc
Nov 14, 1979·Biochemical and Biophysical Research Communications·T A KrulwichA A Guffanti
Feb 8, 1991·Biochimica Et Biophysica Acta·P A Dibrov
Oct 22, 2017·The Journal of General Physiology·Parameswaran Hariharan, Lan Guan
Jun 19, 1989·FEBS Letters·V P Skulachev
Jan 1, 1985·Annals of the New York Academy of Sciences·T TsuchiyaT H Wilson
Jan 1, 1980·Journal of Supramolecular Structure·T H Wilson, E C Lin
Jan 30, 1990·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P Dimroth
Jan 24, 1997·The Journal of Biological Chemistry·B A HirayamaE M Wright
Jan 1, 1985·The Journal of Membrane Biology·C C ChenT H Wilson
Jul 1, 1992·Molecular & General Genetics : MGG·K MizushimaT Tsuchiya
Dec 16, 1998·Journal of Bacteriology·T HiramatsuT Tsuchiya

Citations

May 9, 1977·Biochemical and Biophysical Research Communications·T TsuchiyaT H Wilson
Jan 1, 1977·Reviews of Physiology, Biochemistry and Pharmacology·R K Crane
Sep 9, 1977·Biochemical and Biophysical Research Communications·S M Hasan, T Tsuchiya
Jul 21, 1978·The Journal of Membrane Biology·T TsuchiyaT H Wilson
Dec 15, 1977·The Journal of Membrane Biology·H N Christensen, M E Handlogten
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·C L Slayman, C W Slayman
Oct 1, 1969·Journal of Bacteriology·L Frank, I Hopkins
Nov 9, 1970·Biochemical and Biophysical Research Communications·I C West
Jul 2, 1971·Biochemical and Biophysical Research Communications·J Stock, S Roseman
May 4, 1965·Biochimica Et Biophysica Acta·L S PRESTIDGE, A B PARDEE

Related Concepts

Melibiose
Alkalescens-Dispar Group
Thiogalactosides
Protons
Galactose
RA11
thiomethylgalactoside
Cations
Sodium
Galactosides

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.