Cationic amphiphiles and the solubilization of cholesterol crystallites in membrane bilayers

Biochimica Et Biophysica Acta
Carlos R BenattiRichard M Epand

Abstract

Cationic amphiphiles used for transfection can be incorporated into biological membranes. By differential scanning calorimetry (DSC), cholesterol solubilization in phospholipid membranes, in the absence and presence of cationic amphiphiles, was determined. Two different systems were studied: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)+cholesterol (1:3, POPC:Chol, molar ratio) and 1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-l-serine] (POPS)+cholesterol (3:2, POPS:Chol, molar ratio), which contain cholesterol in crystallite form. For the zwitterionic lipid POPC, cationic amphiphiles were tested, up to 7 mol%, while for anionic POPS bilayers, which possibly incorporate more positive amphiphiles, the fractions used were higher, up to 23 mol%. 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) and DOTAP in methyl sulfate salt form (DOTAPmss) were found to cause a small decrease on the enthalpy of the cholesterol transition of pure cholesterol aggregates, possibly indicating a slight increase on the cholesterol solubilization in POPC vesicles. With the anionic system POPS:Chol, the cationic amphiphiles dramatically change the cholesterol crystal thermal transition, indicating significant changes in the cholesterol aggregates...Continue Reading

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Citations

Sep 27, 2012·Colloids and Surfaces. B, Biointerfaces·Katarzyna Hąc-WydroPatrycja Dynarowicz-Łątka
Jul 10, 2012·Chemistry and Physics of Lipids·C Facundo TempranaM Teresa Lamy
Aug 17, 2011·Biophysical Journal·Laxman MainaliWitold K Subczynski
Oct 26, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Sindhu DoppalapudiWahid Khan
Aug 25, 2017·Biophysics Reviews·Julio H K RozenfeldM Teresa Lamy

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