Cationic and neutral amino acid transporter transcript abundances are differentially expressed in the equine intestinal tract

Journal of Animal Science
A D WoodwardN L Trottier

Abstract

To test the hypothesis that AA transporter transcripts are present in the large intestine and similarly expressed along the intestinal tract, mRNA abundance of candidate AA transporter genes solute carrier (SLC) family 7, member 9 (SLC7A9), SLC7A1, SLC7A8, and SLC43A1 encoding for b(0,+)-type AA transporter (b(0,+)AT), cationic AA transporter-1 (CAT-1), L-type AA transporter-2 (LAT-2), and L-type AA transporter-3 (LAT-3), respectively, was determined in small and large intestinal segments of the horse. Mucosa was collected from the equine small (jejunum and ileum) and large intestine (cecum, left ventral colon, and left dorsal colon), flash frozen in liquid nitrogen, and stored at -80 degrees C. Messenger RNA was isolated from tissue samples, followed by manufacture of cDNA. Relative quantitative reverse transcription-PCR was conducted using the 2(-DeltaDeltaCT) method, with glyceraldehyde-3-phosphate dehydrogenase serving as the housekeeping gene. Compared with the jejunum, cationic and neutral AA transporter SLC7A9 mRNA abundance was similar in the ileum, cecum, and large intestinal segments. Compared with the jejunum, cationic AA transporter SLC7A1 mRNA abundance was similar in the ileum and decreased in the cecum, left vent...Continue Reading

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Citations

Oct 31, 2012·The British Journal of Nutrition·Wouter H HendriksGuido Bosch
Jan 1, 2011·Animal : an International Journal of Animal Bioscience·A S SantosW Martin-Rosset
Feb 9, 2020·Journal of Veterinary Internal Medicine·Jenifer R GoldNicolas F Villarino
Mar 14, 2020·Asian-Australasian Journal of Animal Sciences·Chan Hee Mok, Kristine Laura Urschel
Apr 7, 2021·The Veterinary Clinics of North America. Equine Practice·Patricia Harris, Megan Shepherd
Apr 7, 2021·The Veterinary Clinics of North America. Equine Practice·Kristine L Urschel, Erica C McKenzie
Jun 29, 2021·Translational Animal Science·Patty M Graham-Thiers, LaAnn K Bowen

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