Cationic chitosan-propolis nanoparticles alter the zeta potential of S. epidermidis, inhibit biofilm formation by modulating gene expression and exhibit synergism with antibiotics

PloS One
Teik Hwa OngFabian Davamani

Abstract

Staphylococcus epidermidis, is a common microflora of human body that can cause opportunistic infections associated with indwelling devices. It is resistant to multiple antibiotics necessitating the need for naturally occurring antibacterial agents. Malaysian propolis, a natural product obtained from beehives exhibits antimicrobial and antibiofilm properties. Chitosan-propolis nanoparticles (CPNP) were prepared using Malaysian propolis and tested for their effect against S. epidermidis. The cationic nanoparticles depicted a zeta potential of +40 and increased the net electric charge (zeta potential) of S. epidermidis from -17 to -11 mV in a concentration-dependent manner whereas, ethanol (Eth) and ethyl acetate (EA) extracts of propolis further decreased the zeta potential from -17 to -20 mV. Confocal laser scanning microscopy (CLSM) depicted that CPNP effectively disrupted biofilm formation by S. epidermidis and decreased viability to ~25% compared to Eth and EA with viability of ~60-70%. CPNP was more effective in reducing the viability of both planktonic as well as biofilm bacteria compared to Eth and EA. At 100 μg/mL concentration, CPNP decreased the survival of biofilm bacteria by ~70% compared to Eth or EA extracts which ...Continue Reading

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Citations

Jul 24, 2020·Biotechnology and Applied Biochemistry·Ozgur CeylanHuseyin Cicek
Nov 3, 2020·International Journal of Biological Macromolecules·Lana Glerieide Silva GarciaRaimunda Sâmia Nogueira Brilhante
May 4, 2020·Colloids and Surfaces. B, Biointerfaces·Xixi XiaoMatthew Libera
Mar 3, 2021·Biochimica Et Biophysica Acta. Biomembranes·Anike P V Ferreyra MaillardAxel Hollmann
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Harinash RaoPei Pei Chong
Apr 4, 2021·Polymers·Cai-Ling KeChing-Hsuan Lin
Apr 2, 2021·The Journal of Pharmacy and Pharmacology·Nicolas RipariJosé Maurício Sforcin
Jun 3, 2021·Antibiotics·Anbazhagan SathiyaseelanMyeong-Hyeon Wang
Jun 3, 2021·Biology·Nelly Rivera-YañezOscar Nieto-Yañez
Jul 18, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Menglei WangLi Li
Aug 10, 2021·Colloids and Surfaces. B, Biointerfaces·Maryam TavafoghiMaria Dittrich
Oct 27, 2021·Advanced Drug Delivery Reviews·Sajid AsgharThierry F Vandamme

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Methods Mentioned

BETA
scanning electron microscopy
PCR

Software Mentioned

ImageJ
Leica LAS AF

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