Cationic Conjugated Polymer/Hyaluronan-Doxorubicin Complex for Sensitive Fluorescence Detection of Hyaluronidase and Tumor-Targeting Drug Delivery and Imaging

ACS Applied Materials & Interfaces
Yanqin HuangWei Huang

Abstract

Hyaluronidase (HAase) is becoming a new type of tumor marker since it has been demonstrated to be overexpressed in various kinds of cancer cells. In this study, we described a novel fluorescence method for sensitive, rapid, and convenient HAase detection and tumor-targeting drug delivery and imaging, using a probe prepared by electrostatic assembly of a cationic conjugated polymer (CCP) and anionic hyaluronan (HA) conjugated with the anticancer drug doxorubicin (Dox). The CCP we used was poly{[9,9-bis(6'-(N,N,N-diethylmethylammonium)hexyl)-2,7-fluorenylene ethynylene]-alt-co-[2,5-bis(3'-(N,N,N-diethylmethylammonium)-1'-oxapropyl)-1,4-phenylene]} tetraiodide (PFEP). HA is a natural mucopolysaccharide that can be hydrolyzed by HAase into fragments with low molecular weights. In the PFEP/HA-Dox complex, the fluorescence of PFEP was efficiently quenched due to electron transfer from PFEP to Dox. After the PFEP/HA-Dox complex was exposed to HAase or was taken up by cancer cells through the specific binding between HA and CD44 receptor, HA was degraded by HAase to release the Dox, leading to the recovery of PFEP fluorescence to the "turn-on" state. Moreover, the degree of fluorescence recovery was quantitatively correlated with the c...Continue Reading

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Citations

Oct 28, 2016·ACS Applied Materials & Interfaces·Wen-Ying HuangYu-Hsin Lin
Oct 24, 2017·Physical Chemistry Chemical Physics : PCCP·Lisa PeterhansNatalie Banerji
Sep 27, 2018·Advanced Materials·Zhenjie XueTie Wang
Aug 8, 2019·Expert Opinion on Drug Delivery·Yu Sakurai, Hideyoshi Harashima
Nov 7, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yan WuZhong Liu
May 7, 2021·Analytical Methods : Advancing Methods and Applications·Shenglan WangRongjin Zeng
Dec 15, 2016·ACS Applied Materials & Interfaces·Huijuan ZhangZhenzhong Zhang
Feb 12, 2018·ACS Biomaterials Science & Engineering·Xuezhi ZhaoHua Wei

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