Cationic Dendrimer G2-S16 Inhibits Herpes Simplex Type 2 Infection and Protects Mice Vaginal Microbiome

Pharmaceutics
Carlos Guerrero-BeltranMaria Angeles Muñoz-Fernández

Abstract

The G2-S16 polyanionic carbosilane dendrimer is a promising microbicide that inhibits HSV-2 infection in vitro and in vivo in mice models. This G2-S16 dendrimer inhibits HSV-2 infection even in the presence of semen. Murine models, such as BALB/c female mice, are generally used to characterize host-pathogen interactions within the vaginal tract. However, the composition of endogenous vaginal flora remains largely undefined with modern microbiome analyses. It is important to note that the G2-S16 dendrimer does not change healthy mouse vaginal microbiome where Pseudomonas (10.2-79.1%) and Janthinobacterium (0.7-13%) are the more abundant genera. The HSV-2 vaginally infected female mice showed a significant microbiome alteration because an increase of Staphylococcus (up to 98.8%) and Escherichia (30.76%) levels were observed becoming these bacteria the predominant genera. BALB/c female mice vaginally-treated with the G2-S16 dendrimer and infected with the HSV-2 maintained a healthy vaginal microbiome similar to uninfected female mice. Summarizing, the G2-S16 polyanionic carbosilane dendrimer inhibits the HSV-2 infection in the presence of semen and prevents the alteration of mice female vaginal microbiome.

References

Oct 1, 1977·The Journal of Experimental Zoology·T R KoiterP van der Schoot
Feb 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·W S GallichanK L Rosenthal
Apr 5, 2001·Current Infectious Disease Reports·Jane R. Schwebke
Apr 22, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Thomas L CherpesSharon L Hillier
May 19, 2006·Ecology Letters·Marti J AndersonBrian H McArdle
Jul 26, 2008·Science·Robert M GrantMark A Wainberg
Mar 11, 2009·Retrovirology·Isabel García-MerinoUNKNOWN Spanish HIV BioBank
Apr 9, 2010·Clinical Microbiology Reviews·Jacqueline M Achkar, Bettina C Fries
Dec 3, 2011·The Journal of Clinical Investigation·Rebecca M Brotman
Jun 2, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·L ChoncoM A Muñoz-Fernández
Sep 13, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Beatriz RasinesRafael Gómez
Dec 20, 2012·MBio·Jacques RavelSteven L Zeichner
Jun 25, 2013·FEMS Microbiology Reviews·Mariya I PetrovaSarah Lebeer
Aug 24, 2013·Sexually Transmitted Diseases·Ellen W WiewelLucia V Torian
Sep 13, 2013·Journal of Virology·Qiyi TangYasuhiro Yamamura
Jan 1, 2014·BMC Microbiology·Kenneth Klingenberg BarfodKaren Angeliki Krogfelt
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Nov 14, 2014·Science Translational Medicine·Onofrio ZirafiJan Münch
Jan 16, 2015·The Journal of Infectious Diseases·Allahna EsberAbigail Norris Turner
Mar 13, 2015·Pathogens and Disease·Elizabeth NeuendorfJacques Ravel
May 12, 2015·Nanomedicine : Nanotechnology, Biology, and Medicine·Daniel Sepúlveda-CrespoM Ángeles Muñoz-Fernández
Jan 20, 2016·PloS One·Didiana Jaramillo-RuizM Ángeles Muñoz-Fernández
Jun 5, 2016·Bioinformatics·Vladimir I UlyantsevDmitry G Alexeev
Jun 9, 2016·International Journal of Nanomedicine·Rafael Ceña-DiezM Ángeles Muñoz-Fernández
Dec 23, 2016·Frontiers in Microbiology·Elizabeth A MillerElizabeth A Archie
Mar 10, 2017·Obstetrics and Gynecology·Felicia M T LewisSevgi O Aral
Jun 3, 2017·Science·Nichole R KlattAdam D Burgener
Jun 5, 2017·European Journal of Pharmacology·Pilar García-BroncanoM Ángeles Muñoz-Fernández
Jul 5, 2017·Nanoscale·Rafael Ceña-DiezMªÁngeles Muñoz-Fernández
Aug 16, 2017·Current HIV/AIDS Reports·Jennifer Velloza, Renee Heffron
Nov 15, 2017·Scientific Reports·Supriya D MehtaRobert C Bailey
Feb 10, 2018·AIDS·McKenna C Eastment, R Scott McClelland
May 26, 2018·Nature Reviews. Microbiology·Rob KnightPieter C Dorrestein

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Citations

Aug 30, 2020·International Journal of Pharmaceutics·Matheus Aparecido Dos Santos RamosMarlus Chorilli
Dec 17, 2020·International Journal of Molecular Sciences·Ignacio Relaño-Rodríguez, Maria Ángeles Muñoz-Fernández
Aug 28, 2021·International Journal of Molecular Sciences·Ignacio Relaño-RodríguezMaría Ángeles Muñoz-Fernández
Jan 13, 2022·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Ignacio Rodríguez-IzquierdoMa Ángeles Muñoz-Fernández

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Datasets Mentioned

BETA
PRJNA480951

Methods Mentioned

BETA
FCS
lavage
lavages
Assay

Software Mentioned

Nextera XT
trimmomatic
Permanova
MetaFast
R package Vegan
BLASTn
BMTagger

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