Cationic Europium Complexes for Visualizing Fluctuations in Mitochondrial ATP Levels in Living Cells

Chemistry : a European Journal
Romain MailhotStephen J Butler

Abstract

The ability to study cellular metabolism and enzymatic processes involving adenosine triphosphate (ATP) is impeded by the lack of imaging probes capable of signalling the concentration and distribution of intracellular ATP rapidly, with high sensitivity. We report here the first example of a luminescent lanthanide complex capable of visualizing changes in the concentration of ATP in the mitochondria of living cells. Four cationic europium(III) complexes [Eu.1-4]+ have been synthesized and their binding capabilities towards nucleoside polyphosphate anions examined in aqueous solution at physiological pH. Complexes [Eu.1]+ and [Eu.3]+ bearing hydrogen bond donor groups in the pendant arms showed excellent discrimination between ATP, ADP and monophosphate species. Complex [Eu.3]+ showed relatively strong binding to ATP (logKa =5.8), providing a rapid, long-lived luminescent signal that enabled its detection in a highly competitive aqueous medium containing biologically relevant concentrations of Mg2+ , ADP, GTP, UTP and human serum albumin. This EuIII complex responds linearly to ATP within the physiological concentration range (1-5 mm), and was used to continuously monitor the apyrase-catalyzed hydrolysis of ATP to ADP in vitro. ...Continue Reading

References

Nov 9, 1994·Molecular and Cellular Biochemistry·T W Traut
May 20, 1998·Biophysical Journal·G VerebT M Jovin
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·J LlopisR Y Tsien
Jan 4, 2001·Trends in Neurosciences·R D Fields, B Stevens
May 4, 2002·Annual Review of Biophysics and Biomolecular Structure·Paul R Selvin
Feb 5, 2003·Progress in Biophysics and Molecular Biology·Susumu Seino, Takashi Miki
Sep 27, 2005·Inorganic Chemistry·Loïc J CharbonnièreRaymond F Ziessel
Jan 4, 2007·Journal of the American Chemical Society·Stéphane PetoudKenneth N Raymond
Oct 12, 2007·Journal of the American Chemical Society·Kenjiro HanaokaTetsuo Nagano
Aug 15, 2008·Journal of the American Chemical Society·Akio OjidaItaru Hamachi
Nov 15, 2008·Trends in Neurosciences·Maria P AbbracchioHerbert Zimmermann
Jan 6, 2009·Nature Methods·Jim BergGary Yellen
Feb 5, 2009·Accounts of Chemical Research·Craig P MontgomeryDavid Parker
Mar 4, 2009·Cell Metabolism·Rebeca Acin-PerezGiovanni Manfredi
Mar 28, 2009·Accounts of Chemical Research·Evan G MooreKenneth N Raymond
Sep 2, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hiromi ImamuraHiroyuki Noji
Dec 22, 2009·Chemical Society Reviews·Svetlana V Eliseeva, Jean-Claude G Bünzli
Dec 22, 2009·Chemical Communications : Chem Comm·Vanesa Fernández-MoreiraMichael P Coogan
Mar 15, 2011·Nature·Bing XiaoSteven J Gamblin
Feb 15, 2012·Chemical Communications : Chem Comm·Alla Sreenivasa RaoKyo Han Ahn
Jul 5, 2012·Chemical Society Reviews·Stephen J Butler, David Parker
Sep 22, 2012·Journal of the American Chemical Society·Evan A WeitzValerie C Pierre
Sep 26, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·David J LewisZoe Pikramenou
Jan 22, 2013·Chemical Communications : Chem Comm·James W WaltonDavid Parker
May 22, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Xiao LiuYu Tang
Aug 31, 2013·Chemical Communications : Chem Comm·Kathryn L GempfDavid Parker
Nov 23, 2013·Chemical Communications : Chem Comm·Michael P Coogan, Vanesa Fernández-Moreira
Dec 18, 2013·Chemical Reviews·Marie C HeffernThomas J Meade
Jun 10, 2014·Angewandte Chemie·Tao LiuLoïc J Charbonnière
Oct 15, 2014·Proceedings of the National Academy of Sciences of the United States of America·Elisabeth GoutRichard Bligny

❮ Previous
Next ❯

Citations

Oct 6, 2018·Chemistry : a European Journal·Aleksandr M AgafontsevEvgeny A Kataev
May 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jorge H S K Monteiro
Jun 5, 2020·Chemistry : a European Journal·Aleksandr M AgafontsevEvgeny A Kataev
Nov 1, 2018·Dalton Transactions : an International Journal of Inorganic Chemistry·Laura B JenningsDavid Parker
Jul 16, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wei WangXiliang Luo
Apr 29, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elena Del Giorgio, Thomas Just Sørensen
May 2, 2021·Chemistry : a European Journal·Luis Martínez-CrespoHennie Valkenier
Oct 16, 2020·ChemPlusChem·Stephen J Butler, Katrina A Jolliffe
Jun 25, 2021·Chemical Science·Samantha E Bodman, Stephen J Butler
Jul 7, 2020·Journal of the American Chemical Society·Daniel KovacsK Eszter Borbas
Feb 8, 2019·Journal of the American Chemical Society·Arseni BorissovPaul D Beer

❮ Previous
Next ❯

Methods Mentioned

BETA
biosensors
confocal microscopy

Software Mentioned

ATeam
PercevalHR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.