PMID: 8597610Feb 1, 1996Paper

Cationic lipid mediated transfer of c-abl and bcr antisense oligonucleotides to immature normal myeloid cells: uptake, biological effects and modulation of gene expression

Annals of Hematology
T AlbrechtW E Aulitzky

Abstract

Uptake and biochemical and biological effects of antisense oligodeoxynucleotides (ODN) specific for c-abl and bcr genes were studied in normal immature myeloid cells. CD34-positive cells were purified by positive and negative selection and cultured in liquid culture for 7 days. These cells were then incubated with ODNs, either alone or in combination with cationic lipids. The uptake of ODNs was enhanced by the use of cationic lipids. In addition, very low concentrations of ODNs in combination with cationic lipids were capable of specifically inhibiting the expression of the c-abl gene. In contrast, no effects were seen on the expression of bcr. However, despite the effective blocking of c-abl expression, no changes in cellular growth patterns were observed in liquid culture or in a colony-forming assay. We conclude tht the use of cationic lipids might enhance the gene-regulatory effects of ODNs by increasing their uptake into normal hematopoietic cells.

References

Jun 21, 1990·Biochimica Et Biophysica Acta·C Hélène, J J Toulmé
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J P LeonettiB Lebleu
Mar 1, 1994·European Journal of Haematology·A M LiberatiG Saglio
Dec 15, 1993·Biochemical and Biophysical Research Communications·S CapaccioliA Quattrone
Jan 1, 1993·Molecular and Cellular Biology·G KellerM V Wiles

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Citations

Feb 5, 1998·Antisense & Nucleic Acid Drug Development·W BileckiR Przewłocki
Feb 12, 1998·Biochemical and Molecular Medicine·Y LavrovskyA K Roy

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