Cationic liposome-mediated gene delivery to the liver and to hepatocellular carcinomas in mice

Human Gene Therapy
L MohrJ R Wands

Abstract

The potential of cationic liposomes as nonviral vectors for in vivo gene delivery to the liver and to intrahepatic hepatocellular carcinoma (HCC) was investigated. Mice were injected via the tail vein or portal vein with a cationic lipid complexed to plasmid DNA (pDNA) encoding the chloramphenicol acetyltransferase (CAT) reporter gene at various cationic lipid:pDNA molar ratios to analyze the efficiency of gene delivery after intravenous administration. Tail vein injection resulted in high CAT expression levels in lung and spleen and low levels in the liver. Portal vein injection, by comparison, significantly enhanced hepatic reporter gene expression but also resulted in pronounced hepatic toxicity. Gene delivery to intrahepatic tumors produced by intrahepatic injection of human HCC cells was analyzed in nude mice. Tail vein injection as well as portal vein injection resulted in low levels of gene expression in intrahepatic tumors. By comparison, high levels of gene expression were achieved by direct, intratumoral injection of liposome-pDNA complexes, with only minimal expression in the surrounding normal liver. Therefore, direct liposome-pDNA complex injection appears far superior to systemic or portal intravenous administrati...Continue Reading

References

Nov 18, 1991·Biochimica Et Biophysica Acta·J H SeniorR Maskiewicz
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard
Oct 20, 1995·The Journal of Biological Chemistry·Y LiuR Debs
Mar 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M A KayS L Woo
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·J S RemyJ P Behr
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·J F EngelhardtJ M Wilson
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·J C PeralesR W Hanson
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y YangJ M Wilson
May 14, 1993·Science·R C Mulligan
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·M CarusoS H Chen
Oct 1, 1996·Human Gene Therapy·P L Felgner
Sep 26, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S TanakaJ R Wands
Jan 13, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K OhnishiK Fujiwara
Apr 29, 1998·The Journal of Clinical Investigation·G ThurstonD M McDonald
Dec 24, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·L MohrJ R Wands

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Citations

Jun 3, 2004·Biomaterials·Woo-Jeong ChoiChong-Kook Kim
Jun 28, 2002·International Journal of Pharmaceutics·Crispin R Dass
Dec 14, 2002·The International Journal of Biochemistry & Cell Biology·Bruno SangroJesus Prieto
Dec 11, 2002·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Betsy T KrenClifford J Steer
Nov 7, 2008·Cancer Biotherapy & Radiopharmaceuticals·Yong Nan JinJune-Key Chung
Feb 19, 2002·Expert Opinion on Biological Therapy·Leonhard MohrHubert E Blum
Oct 20, 2006·Journal of Drug Targeting·Conchita Tros De IlarduyaNejat Düzgüneş
Mar 24, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Long XuThomas J Anchordoquy
Mar 6, 2012·Journal of Vascular and Interventional Radiology : JVIR·Young Il KimLawrence V Hofmann
Jan 18, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Masateru MiyakeToshikiro Kimura
May 15, 2002·The Journal of Pharmacy and Pharmacology·Crispin R Dass
Apr 12, 2003·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Akira YamaguchiShoji Yamanaka
Feb 20, 2016·Biomaterials Science·Liu YangJiaoyan Ren
Jul 9, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jian WuMark A Zern
Aug 29, 2003·Cancer Gene Therapy·René GérolamiClaude Bagnis
Feb 11, 2004·Endocrine Reviews·Luisa BarzonGiorgio Palù
Aug 20, 2019·Journal of Liposome Research·Pirthi Pal SinghRavinder Kodipyaka
Apr 3, 2020·Biological & Pharmaceutical Bulletin·Ikramy A KhalilHideyoshi Harashima
Jun 13, 2021·Journal of Pharmaceutical Sciences·Masateru MiyakeKazutaka Higaki
Mar 26, 2019·ACS Nano·Jonas BuckDominik Witzigmann

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