Cationic peptides as RNA compaction agents: a study on the polyA compaction activity of a linear alpha,epsilon-oligo-L-lysine

International Journal of Pharmaceutics
Giovanni N RovielloValentina Roviello

Abstract

In this work, we investigate the compaction activity of a sequential alpha,epsilon-peptide composed of l-lysines towards two RNA targets, in view of its possible pharmaceutical application in RNA-targeting and RNA delivery. The basic oligolysine, object of the present study, proved not only to be efficient in compacting the single-stranded polyA RNA, but also to strongly interact with the polyA·polyU complex, as evidenced by CD-binding and UV-melting experiments. In particular, the marked differences in the CD spectra of the RNA targets upon addition of the peptide, as well as the different UV melting behaviour for the polyA·polyU complex in the presence and absence of the peptide, sustain the hypothesis of a strong RNA compaction capacity of the alpha,epsilon-oligolysine. Finally, by using HPLC analysis, we found a good resistance of the peptide against the lytic action of human serum, an important requirement in view of in vitro/in vivo biological assays.

References

May 18, 1995·Biochimica Et Biophysica Acta·C AmatoR Felicioli
Mar 1, 2008·The Journal of Physical Chemistry. B·Simona SennatoMassimo Cametti
Aug 11, 2010·Chemical Communications : Chem Comm·Guangtao Song, Jinsong Ren
Jun 5, 2013·Biological Chemistry·Giovanni N RovielloCarlo Pedone
Jan 15, 2014·Bioorganic & Medicinal Chemistry·Giovanni N RovielloCarlo Pedone
Mar 1, 2014·Biochimica Et Biophysica Acta·Pasqualina Liana ScognamiglioDaniela Marasco
Jul 6, 2014·Journal of Medicinal Chemistry·Alfonso CarotenutoPaolo Grieco

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Citations

Mar 15, 2016·RSC Advances·Giovanni N RovielloMichele Saviano
Apr 30, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·Domenica MusumeciDaniela Montesarchio
Jul 23, 2015·Beilstein Journal of Nanotechnology·Giovanni N RovielloMerab Mirtskhulava
Sep 14, 2018·International Journal of Molecular Sciences·Sara La MannaDaniela Marasco
Nov 20, 2016·Amino Acids·Giovanni N RovielloCaterina Vicidomini

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