CatM regulation of the benABCDE operon: functional divergence of two LysR-type paralogs in Acinetobacter baylyi ADP1

Applied and Environmental Microbiology
Obidimma C EzezikaEllen L Neidle

Abstract

Two LysR-type transcriptional regulators, BenM and CatM, control benzoate consumption by the soil bacterium Acinetobacter baylyi ADP1. These homologs play overlapping roles in the expression of multiple genes. This study focuses on the benABCDE operon, which initiates benzoate catabolism. At this locus, BenM and CatM each activate transcription in response to the catabolite cis,cis-muconate. BenM, but not CatM, additionally responds to benzoate as an effector. Regulation by CatM alone is insufficient for growth on benzoate as the sole carbon source. However, three point mutations independently increased CatM-activated benA transcription and enabled growth on benzoate without BenM. Two mutations generate variants with one amino acid change in the 303-residue CatM, CatM(V158M) and CatM(R156H). These substitutions affected regulation of benA differently than that of catB, another CatM-regulated gene involved in benzoate catabolism. In relation to CatM, CatM(V158M) increased cis,cis-muconate-dependent transcription of benA but decreased that of catB. CatM(R156H) increased effector-independent expression of catB compared to CatM. In contrast, cis,cis-muconate was required with CatM(R156H) to activate unusually high benA expression. ...Continue Reading

References

Oct 1, 1990·Journal of Bacteriology·L A Gregg-Jolly, L N Ornston
Apr 1, 1969·Journal of Bacteriology·E Juni, A Janik
Jan 1, 1993·Annual Review of Microbiology·M A Schell
Mar 6, 2002·The Journal of Biological Chemistry·Reiko Akakura, Stephen C Winans
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Becky M BundyEllen L Neidle
Dec 20, 2003·Acta Crystallographica. Section D, Biological Crystallography·Todd ClarkCory Momany
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·David Tropel, Jan Roelof van der Meer
Oct 19, 2004·Annual Review of Microbiology·Andrew B Reams, Ellen L Neidle
Jan 5, 2005·Archives of Biochemistry and Biophysics·Hoo Hwi ParkHae Ja Shin
Jan 5, 2006·Applied and Environmental Microbiology·Mario VaneechoutteLenie Dijkshoorn

❮ Previous
Next ❯

Citations

Feb 4, 2012·The Journal of Biological Chemistry·J Andrés ValderramaEduardo Díaz
Jun 15, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Obidimma C EzezikaCory Momany
Nov 7, 2006·Applied and Environmental Microbiology·Simone Yasmin SiehlerUlrike Gerischer
Apr 30, 2009·Molecular Microbiology·Sarah H CravenEllen L Neidle
Feb 13, 2007·Journal of Molecular Biology·Obidimma C EzezikaCory Momany
Dec 3, 2008·Microbiology·Sarah E Maddocks, Petra C F Oyston
Feb 4, 2016·Angewandte Chemie·Miguel SuasteguiJean-Philippe Tessonnier
Oct 9, 2013·Acta Crystallographica. Section D, Biological Crystallography·Amer M AlanaziCory Momany
Apr 16, 2019·Frontiers in Microbiology·Inna A Suvorova, Mikhail S Gelfand
Jan 14, 2020·Metabolic Engineering·Gayle J BentleyGregg T Beckham
Jan 15, 2020·ACS Synthetic Biology·Francesca AmbriJay D Keasling

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.