CatroxMP-II: a heme-modulated fibrinogenolytic metalloproteinase isolated from Crotalus atrox venom

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Montamas SuntravatVance G Nielsen

Abstract

It has been recently demonstrated that the hemotoxic venom activity of several species of snakes can be inhibited by carbon monoxide (CO) or a metheme forming agent. These and other data suggest that the biometal, heme, may be attached to venom enzymes and may be modulated by CO. A novel fibrinogenolytic metalloproteinase, named CatroxMP-II, was isolated and purified from the venom of a Crotalus atrox viper, and subjected to proteolysis and mass spectroscopy. An ion similar to the predicted singly charged m/z of heme at 617.18 was identified. Lastly, CORM-2 (tricarbonyldichlororuthenium (II) dimer, a CO releasing molecule) inhibited the fibrinogenolytic effects of CatroxMP-II on coagulation kinetics in human plasma. In conclusion, we present the first example of a snake venom metalloproteinase that is heme-bound and CO-inhibited.

References

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Apr 1, 2011·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Vance G NielsenKeith Vosseller
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Aug 6, 2016·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Vance G NielsenQuinlan Amos
Aug 23, 2016·Basic & Clinical Pharmacology & Toxicology·Vance G Nielsen, Philip A Losada
Oct 28, 2016·Journal of Thrombosis and Thrombolysis·Vance G Nielsen, Charles M Bazzell
Jul 12, 2017·Basic & Clinical Pharmacology & Toxicology·Vance G Nielsen
Nov 25, 2017·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Vance G NielsenRyan W Matika

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Citations

May 28, 2019·Journal of Thrombosis and Thrombolysis·Vance G Nielsen
Aug 16, 2019·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Vance G NielsenBrian J Turchioe
Aug 23, 2018·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Vance G Nielsen, Nathaniel Frank
Apr 8, 2019·Journal of Thrombosis and Thrombolysis·Vance G Nielsen, Nathaniel Frank
May 1, 2021·International Journal of Molecular Sciences·Vance G Nielsen
Jun 3, 2021·Toxins·Anant DeshwalSuresh Kumar Thallapuranam

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

XCalibur
OrigenPro
SVMP
Basic Local Alignment Search Tool ( BLAST

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