Cattle and sheep use different promoters to direct the expression of the aromatase cytochrome P450 encoding gene, Cyp19, during pregnancy

Domestic Animal Endocrinology
J VanselowM Schwerin

Abstract

Gestagens and oestrogens are important regulators of pregnancy and parturition. The aim of the present study was the comparative quantification of steroidogenic transcripts in placenta and corpus luteum of cattle and sheep during pregnancy and post partum. Cyp19 transcript variants, derived from different promoters, as well as transcripts of Hsd3b, Cyp11A1, and Cyp17, encoding the steroidogenic enzymes P450arom, 3beta-HSD, P450SCC, and P450C17, respectively, were quantified by newly developed real-time PCR assays. All steroidogenic transcripts were detected in ovine and bovine corpus luteum and placenta during pregnancy, however at a very different concentration. In both species Cyp11A1 and especially Hsd3b transcripts predominated in corpus luteum, outnumbering transcripts of Cyp17 and Cyp19 by more than two and three orders of magnitude, respectively. Cyp19 transcript were found at high concentration in the placenta and at a very low concentration in corpus luteum. Cyp17 transcripts had a relatively low concentration in both, placenta and corpus luteum, however showed a peak of expression in the ovine and bovine term placenta. Tissue- and species-specific Cyp19 transcripts derived from different promoters were detected. In or...Continue Reading

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Citations

Jun 21, 2014·BMC Research Notes·Marina Chwalisz, Rainer Fürbass
Apr 13, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Mónica P RecabarrenSergio E Recabarren
Jul 19, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Xiaofeng ZhengPaul D Carrière
May 17, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Richard G LeaJacqueline M Wallace
Jul 12, 2008·Annual Review of Cell and Developmental Biology·Saara M Rawn, James C Cross
Jul 1, 2009·Genetics and Molecular Biology·Ana Maria Bezerra Oliveira LôboSamuel Rezende Paiva

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