Cauliflower mosaic virus P6 inclusion body formation: A dynamic and intricate process

Virology
Roberto Alers-VelazquezScott M Leisner

Abstract

During an infection, Cauliflower mosaic virus (CaMV) forms inclusion bodies (IBs) mainly composed of viral protein P6, where viral activities occur. Because viral processes occur in IBs, understanding the mechanisms by which they are formed is crucial. FL-P6 expressed in N. benthamiana leaves formed IBs of a variety of shapes and sizes. Small IBs were dynamic, undergoing fusion/dissociation events. Co-expression of actin-binding polypeptides with FL-P6 altered IB size distribution and inhibited movement. This suggests that IB movement is required for fusion and growth. A P6 deletion mutant was discovered that formed a single large IB per cell, which suggests it exhibited altered fusion/dissociation dynamics. Myosin-inhibiting drugs did not affect small IB movement, while those inhibiting actin polymerization did. Large IBs colocalized with components of the aggresome pathway, while small ones generally did not. This suggests a possible involvement of the aggresome pathway in large IB formation.

References

Jan 1, 1977·Advances in Virus Research·G P Martelli, M Russo
Jan 1, 1976·Advances in Virus Research·R J Shepherd
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S GowdaR J Shepherd
Aug 25, 1989·Nucleic Acids Research·D MattanovichH Katinger
Jul 1, 1986·The Journal of General Virology·U MelcherR C Essenberg
Oct 1, 1971·The Journal of General Virology·G P Martelli, M A Castellano
Feb 1, 1994·Current Opinion in Cell Biology·L P CramerJ A Theriot
Dec 29, 1998·The Journal of Cell Biology·J A JohnstonR R Kopito
Sep 24, 1999·The Journal of Cell Biology·R García-MataE S Sztul
Dec 21, 2000·Trends in Cell Biology·R R Kopito
Nov 20, 2002·Molecular Plant-microbe Interactions : MPMI·Yongzhong Li, Scott M Leisner
Jun 19, 2004·The Journal of Biological Chemistry·Mihály KovácsJames R Sellers
Jan 20, 2005·Biology of the Cell·Reyes R NovoaCristina Risco
Apr 6, 2007·The EMBO Journal·Sang Hyon KimMichael Taliansky
Sep 28, 2007·Annual Review of Microbiology·Thomas Wileman
Dec 17, 2008·The Plant Journal : for Cell and Molecular Biology·Alexandre MartinièreMartin Drucker
Nov 1, 2002·Molecular Plant Pathology·Muriel HaasMario Keller
Oct 28, 2010·Molecular Plant-microbe Interactions : MPMI·Carlos A AngelJames E Schoelz
Oct 5, 2011·The Journal of Cell Biology·Cat M Haglund, Matthew D Welch
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Sep 14, 2012·Frontiers in Plant Science·Motoki Tominaga, Akihiko Nakano
Sep 18, 2012·Cellular Microbiology·Isabel Fernández de CastroCristina Risco
Dec 4, 2012·Viruses·Adi Moshe, Rena Gorovits

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