Cav3.2 T-type calcium channels shape electrical firing in mouse Lamina II neurons

Scientific Reports
Miriam CandelasPierre-François Méry

Abstract

The T-type calcium channel, Cav3.2, is necessary for acute pain perception, as well as mechanical and cold allodynia in mice. Being found throughout sensory pathways, from excitatory primary afferent neurons up to pain matrix structures, it is a promising target for analgesics. In our study, Cav3.2 was detected in ~60% of the lamina II (LII) neurons of the spinal cord, a site for integration of sensory processing. It was co-expressed with Tlx3 and Pax2, markers of excitatory and inhibitory interneurons, as well as nNOS, calretinin, calbindin, PKCγ and not parvalbumin. Non-selective T-type channel blockers slowed the inhibitory but not the excitatory transmission in LII neurons. Furthermore, T-type channel blockers modified the intrinsic properties of LII neurons, abolishing low-threshold activated currents, rebound depolarizations, and blunting excitability. The recording of Cav3.2-positive LII neurons, after intraspinal injection of AAV-DJ-Cav3.2-mcherry, showed that their intrinsic properties resembled those of the global population. However, Cav3.2 ablation in the dorsal horn of Cav3.2GFP-Flox KI mice after intraspinal injection of AAV-DJ-Cav3.2-Cre-IRES-mcherry, had drastic effects. Indeed, it (1) blunted the likelihood of ...Continue Reading

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Citations

Jul 3, 2019·Channels·Francina Agosti, Christophe Altier
Jun 26, 2020·Physiological Reviews·Nanna Brix FinnerupTroels Staehelin Jensen
Jun 30, 2019·Disease Models & Mechanisms·Lucie ValekIrmgard Tegeder
Feb 29, 2020·Journal of Neural Transmission·Alain ArtolaRadhouane Dallel
Sep 3, 2020·Molecular Brain·Vinicius M GadottiGerald W Zamponi
Sep 26, 2020·Biotechnology Journal·Jihad El Andari, Dirk Grimm
Mar 13, 2021·Progress in Neurobiology·Rabia Bouali-BenazzouzPascal Fossat
Apr 20, 2021·British Journal of Pharmacology·Erika K HardingMichael E Hildebrand
Oct 9, 2021·Pflügers Archiv : European journal of physiology·Maria A GandiniGerald W Zamponi

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Methods Mentioned

BETA
PCR
dissection

Software Mentioned

Prism
PrimerBlast
Blastn

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