Caveolae as potential mediators of MCH-signaling pathways

Biochemical and Biophysical Research Communications
Laurie B CookKelsi Robinson

Abstract

The melanin-concentrating hormone receptor (MCHR) 1 is a G protein-coupled receptor involved in the regulation of appetite and energy expenditure in mammals. Here, we show that MCHR1 partitions to lipid rafts in stably expressing Chinese hamster ovary cells. In addition to co-fractionating with lipid rafts containing caveolin-1 on sucrose gradients, caveolin-1 was present in MCHR1 immunoprecipitates, suggesting that MCHR1 complexes with caveolae. The cholesterol-depleting drug methyl-beta-cyclodextrin impaired MCH-mediated ERK signaling. These data suggest that a functional interaction between MCHR1 and caveolin-1 in lipid rafts exists and provide a basis for further biochemical studies to understand the significance on MCH-mediated signal transduction events.

References

Jun 1, 1983·General and Comparative Endocrinology·B I Baker, T A Rance
Mar 29, 2001·The Journal of Biological Chemistry·J HillA I Muir
May 18, 2001·Biochemical and Biophysical Research Communications·M MoriM Fujino
Jun 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·A W SailerQ Liu
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·S AnK Dai
Sep 5, 2001·The Journal of Biological Chemistry·R S OstromP A Insel
May 25, 2002·The Journal of Biological Chemistry·Chang-Cheng ZhuPatricia M Hinkle
Aug 10, 2002·American Journal of Physiology. Endocrinology and Metabolism·Richard L BradleyBentley Cheatham
Mar 18, 2004·The Biochemical Journal·Laurie B Cook, Patricia M Hinkle
Apr 10, 2004·Journal of Molecular Endocrinology·B Chini, M Parenti
Sep 24, 2004·Physiological Reviews·Alex W CohenMichael P Lisanti
Dec 8, 2006·Trends in Cell Biology·Brian P Head, Paul A Insel
Oct 5, 2007·Annual Review of Pharmacology and Toxicology·Hemal H PatelPaul A Insel

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Citations

Dec 19, 2013·International Journal of Endocrinology·Jay I ModenLaurie B Cook
Feb 23, 2010·Molecular and Cellular Endocrinology·Laurie B CookScott Portwood
Mar 2, 2013·The International Journal of Neuropsychopharmacology·Li-Li SunJie Shi
Nov 19, 2010·Journal of Receptor and Signal Transduction Research·Alex N EberleUrs Zumsteg
Feb 11, 2010·Current Opinion in Clinical Nutrition and Metabolic Care

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