Caveolin-1 aggravates cigarette smoke extract-induced MUC5AC secretion in human airway epithelial cells

International Journal of Molecular Medicine
Qiao YuChengping Hu

Abstract

Airway mucus hypersecretion is a major pathological characteristic of chronic obstructive pulmonary disease (COPD), and cigarette smoke is highly implicated in mucus secretion and the development of COPD. Cigarette smoke reportedly induces mucin overproduction through the epidermal growth factor receptor (EGFR) in the airway epithelium; however, the underlying mechanisms responsible for the activation of EGFR remain unknown. Caveolin-1, a component protein in the cytomembrane, reportedly regulates airway inflammation and lung injury. In this study, we aimed to determine whether caveolin-1 modulates mucin hyperproduction induced by cigarette smoke. Our results revealed that cigarette smoke extract (CSE) significantly increased MUC5AC production, as well as the levels of phosphorylated EGFR (p-EGFR) and phosphorylated Akt (p-Akt) in human bronchial epithelial cells (16HBE cells), as shown by ELISA, RT-PCR and western blot analysis. These effects were prevented by treatment with EGFR inhibitor (AG1478) and phosphatidylinostol-3-kinase (PI3K) inhibitor (LY294002). We also found that the overexpression of caveolin-1 enhanced the expression of MUC5AC, p-EGFR and p-Akt induced by CSE. Conversely, the downregulation of caveolin-1 by si...Continue Reading

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Citations

Jan 29, 2019·Expert Review of Respiratory Medicine·Sarah A WicherChristina M Pabelick
Apr 6, 2018·International Journal of Molecular Medicine·Lingling TangXiongbiao Wang
Feb 8, 2019·BMC Pulmonary Medicine·Qiao YuQiong Chen
May 8, 2021·The Journal of Pathology·Sharif MoradiMostafa Ghanei
Jul 25, 2021·International Journal of Molecular Sciences·Stanislav Kotlyarov, Anna Kotlyarova

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