Caveolin-1, Id3a and two LIM protein genes are upregulated by estrogen in vascular smooth muscle cells

Life Sciences
Tokumitsu WatanabeYasuyoshi Ouchi

Abstract

Estrogen has diverse effects on the vasculature, such as vasodilation, endothelial growth and inhibition of vascular smooth muscle cell (VSMC) proliferation and migration. However, little is known about the genes that are regulated by estrogen in the vascular wall. Wistar rats were ovariectomized or sham-operated (Sham group), and 2 weeks after the operation, were subjected to subcutaneous implantation of placebo pellets (OVX + V group) or estradiol pellets (OVX + E group). Endothelium-denuded aortic tissue was examined 2 weeks after implantation. By applying high-density oligonucleotide microarray analysis, the expression of approximately 7000 genes was analyzed. Among the genes with different expression levels between the OVX + E group and the OVX + V group, those that have been reported to be expressed in the vasculature or muscle tissue, were chosen. Finally, four genes, caveolin-1, two LIM proteins (enigma and SmLIM) and Id3a, were identified. Microarray as well as real-time polymerase chain reaction showed that the expression levels of these genes were significantly higher in the OVX + E group than in the OVX + V group. To clarify whether estrogen directly upregulates these genes in the vascular wall, Northern blot analys...Continue Reading

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Citations

Jun 1, 2006·Biochemical and Biophysical Research Communications·Jens HerrmannRalf Weiskirchen
Mar 18, 2015·Women's Health·Ronée E HarveyVirginia M Miller
Feb 19, 2013·Biochimica Et Biophysica Acta·T PiraniA Vieira
Jan 1, 2013·Journal of Molecular and Cellular Cardiology·Bo-Kyung SonYasuyoshi Ouchi
Jul 30, 2016·Arteriosclerosis, Thrombosis, and Vascular Biology·Ljubica Perisic MaticUlf Hedin
Jan 13, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Muthuvel Jayachandran, Virginia M Miller
Nov 9, 2006·Acta Biochimica Et Biophysica Sinica·Fa-Lin YangQin Hu

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