Caveolin-1 is a modulator of fibroblast activation and a potential biomarker for gastric cancer

International Journal of Biological Sciences
Xiao-Jun ShenJian-Wei Bi

Abstract

Stromal fibroblasts play an important role in chronic cancer-related inflammation and the development as well as progression of malignant diseases. However, the difference and relationship between inflammation-associated fibroblasts (IAFs) and cancer-associated fibroblasts (CAFs) are poorly understood. In this study, gastric cancer-associated fibroblasts (GCAFs) and their corresponding inflammation-associated fibroblasts (GIAFs) were isolated from gastric cancer (GC) with chronic gastritis and cultured in vitro. These activated fibroblasts exhibited distinct secretion and tumor-promoting behaviors in vitro. Using proteomics and bioinformatics techniques, caveolin-1 (Cav-1) was identified as a major network-centric protein of a sub-network consisting of 121 differentially expressed proteins between GIAFs and GCAFs. Furthermore, immunohistochemistry in a GC cohort showed significant difference in Cav-1 expression score between GIAFs and GCAFs and among patients with different grades of chronic gastritis. Moreover, silencing of Cav-1 in GIAFs and GCAFs using small interfering RNA increased the production of pro-inflammatory and tumor-enhancing cytokines and chemokines in conditioned mediums that elevated cell proliferation and mig...Continue Reading

Citations

May 28, 2016·Antioxidants & Redox Signaling·Géraldine GentricFatima Mechta-Grigoriou
Mar 11, 2018·International Journal of Cancer. Journal International Du Cancer·Julia Ketteler, Diana Klein
Sep 15, 2016·Cancer Informatics·Wenying YanGuang Hu
Oct 9, 2019·International Journal of Molecular Sciences·Mayra Paolillo, Sergio Schinelli
Jul 25, 2017·Journal of Clinical Pathology·Joe YeongPuay Hoon Tan
Oct 13, 2016·World Journal of Gastroenterology : WJG·Changwon KangJ Eugene Lee
Jun 1, 2018·Analytical Cellular Pathology (Amsterdam)·Wladimir GerstenbergerHarriet Wikman
Aug 23, 2017·Gastroenterology Research and Practice·Der Sheng SunYoon Ho Ko
May 27, 2017·Oxidative Medicine and Cellular Longevity·Shengqi WangZhiyu Wang

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Methods Mentioned

BETA
FCS
ELISA
Antibody
transfections
transfection

Software Mentioned

APEX
- Quantitative Proteomics Tool
Bioworks Browser
SPSS
ScanAlyze
GATHER
NetBox

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