Caveolin-3-anchored microdomains at the rabbit sarcoplasmic reticulum membranes

Biochemical and Biophysical Research Communications
Chunlei LiXujia Zhang

Abstract

It is generally believed that sphingomyelin- and cholesterol-enriched microdomains can be isolated as detergent-resistant membranes (DRMs) from plasma membrane and organelle membranes. Here, we describe the isolation and characterization of microdomains from sarcoplasmic reticulum (SR) membranes. These SR-derived detergent-resistant membranes (SR-DRMs) enriched in sphingomyelin and cholesterol have a low buoyant density. Immunofluorescence microscopy of SR membranes shows the presence of caveolin-3 in the SR, known as a marker protein of caveolae at plasma membrane. We also demonstrated that significant amount of SERCAs together with caveolin-3 associates with SR-DRMs and are fully functional. Depletion of cholesterol caused the disruption of SR-DRMs.

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Citations

Jun 3, 2011·World Journal of Gastroenterology : WJG·Angela Dolganiuc
Mar 18, 2015·Pharmacology & Therapeutics·Lucy MurfittAshraf Kitmitto
Nov 15, 2007·Experimental Cell Research·Mika KaakinenKalervo Metsikkö
Jul 17, 2007·Gastroenterology·Jun NagataMichael H Nathanson
Aug 19, 2009·Journal of Neurochemistry·Jianfeng WangRobert W Ledeen
Nov 25, 2006·The International Journal of Biochemistry & Cell Biology·Gabriela KrastevaPeter König

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